Overview

  • Product name

  • Description

    Rabbit polyclonal to MTIF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MTIF2 aa 497-646.

  • Positive control

    • Human cell line A431, Human plasma lysate, Human nasopharynx tissue.
  • General notes

     This product was previously labelled as IF-2(Mt)

     

Properties

Applications

Our Abpromise guarantee covers the use of ab121243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following is recommended: cell fixation with ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permealization with 0.1% Triton X-100 in PBS for 3x5 minutes.
WB 1/250 - 1/500.

Target

  • Function

    One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.
  • Tissue specificity

    Expressed in all tissues examined. Highest level in skeletal muscle.
  • Sequence similarities

    Belongs to the IF-2 family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • IF-2(Mt) antibody
    • IF-2Mt antibody
    • IF2(mt) antibody
    • IF2M_HUMAN antibody
    • mitochondrial antibody
    • mitochondrial translational initiation factor 2 antibody
    • Mtif2 antibody
    • Translation initiation factor IF-2 antibody
    • Translation initiation factor IF-2, mitochondrial antibody
    see all

Images

  • All lanes : Anti-MTIF2 antibody (ab121243) at 1/250 dilution

    Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human liver lysate
    Lane 6 : Human tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human nasopharynx tissue, using 1/25 ab121243, shows strong membranous positivity in respiratory epithelial cells.
  • Immunofluorescent staining, using 4 µg/ml ab121243, in Human cell line A431 shows positivity in mitochondria. Antibody staining is shown in green.

References

ab121243 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121243.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up