Key features and details
- Rabbit polyclonal to MTIF3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MTIF3 antibody
See all MTIF3 primary antibodies
DescriptionRabbit polyclonal to MTIF3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human MTIF3 aa 1-278.
MAALFLKRLTLQTVKSENSCIRCFGKHILQKTAPAQLSPIASAPRLSFLI HAKAFSTAEDTQNEGKKTKKNKTAFSNVGRKISQRVIHLFDEKGNDLGNM HRANVIRLMDERDLRLVQRNTSTEPAEYQLMTGLQILQERQRLREMEKAN PKTGPTLRKELILSSNIGQHDLDTKTKQIQQWIKKKHLVQITIKKGKNVD VSENEMEEIFHQILQTMPGIATFSSRPQAVQGGKALMCVLRAFSKNEEKA YKETQETQERDTLNKDHGNDKESNVLHQ
Database link: Q9H2K0
- WB: Mouse small intestine and liver lysates; Rat heart lysates; HeLa whole cell lysate. IHC-P: Human prostate cancer and lung cancer tissues.
This product was previously labelled as Mitocondrial Translational Initiation Factor 3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 32 kDa.|
FunctionIF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.
Sequence similaritiesBelongs to the IF-3 family.
- Information by UniProt
- DC38 antibody
- IF 3 antibody
- IF 3Mt antibody
All lanes : Anti-MTIF3 antibody (ab230321) at 1/1000 dilution
Lane 1 : Mouse small intestine lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse liver lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32 kDa
Paraffin-embedded human prostate cancer tissue stained for MTIF3 using ab230321 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human lung cancer tissue stained for MTIF3 using ab230321 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230321 has been referenced in 1 publication.
- Song J & Wang C Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops. BMC Genomics 20:363 (2019). PubMed: 31072381