Key features and details
- Rabbit polyclonal to MTMR1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MTMR1 antibody
DescriptionRabbit polyclonal to MTMR1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MTMR1 aa 615-665. The exact sequence is proprietary.
Database link: Q13613
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab240569 was affinity purified using an epitope specific to MTMR1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab240569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 75 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceMTMR1 is a member of the myotubularin related family of proteins. Members of this family contain the consensus sequence for the active site of protein tyrosine phosphatases. The MTMR1 gene shares structural similarities with the MTM1 gene, and is also located in the same chromosomal region. Myotubularin related proteins are a large subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3 phosphorylated inositol lipids. MTMR1 is a lipid phosphatase that acts on phosphatidylinositol 3-phosphate and phosphatidylinositol (3,5)-bisphosphate.
Cellular localizationPlasma Membrane; Peripheral membrane protein, cytoplasmic side.
- MTMR 1 antibody
- Myotubularin related 1 antibody
- Myotubularin related protein 1 antibody
All lanes : Anti-MTMR1 antibody (ab240569) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
MTMR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. For western blotting, ab240569 was used at 0.4 µg/ml.
Lane 1: ab240569 used for IP at 6 µg per reaction.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240569 has not yet been referenced specifically in any publications.