Overview

  • Product name

    Anti-MTMR1 antibody
  • Description

    Rabbit polyclonal to MTMR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MTMR1 aa 615-665. The exact sequence is proprietary.
    Database link: Q13613

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab240569 was affinity purified using an epitope specific to MTMR1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    MTMR1 is a member of the myotubularin related family of proteins. Members of this family contain the consensus sequence for the active site of protein tyrosine phosphatases. The MTMR1 gene shares structural similarities with the MTM1 gene, and is also located in the same chromosomal region. Myotubularin related proteins are a large subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3 phosphorylated inositol lipids. MTMR1 is a lipid phosphatase that acts on phosphatidylinositol 3-phosphate and phosphatidylinositol (3,5)-bisphosphate.
  • Cellular localization

    Plasma Membrane; Peripheral membrane protein, cytoplasmic side.
  • Database links

  • Alternative names

    • MTMR 1 antibody
    • Myotubularin related 1 antibody
    • Myotubularin related protein 1 antibody

Images

  • All lanes : Anti-MTMR1 antibody (ab240569) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 75 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN lysis buffer.

  • MTMR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. For western blotting, ab240569 was used at 0.4 µg/ml.


    Lane 1: ab240569 used for IP at 6 µg per reaction.
    Lane 2: Control IgG.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

ab240569 has not yet been referenced specifically in any publications.

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