Key features and details
- Rabbit polyclonal to mTOR
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-mTOR antibody
See all mTOR primary antibodies
DescriptionRabbit polyclonal to mTOR
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic 15 amino acid peptide from the amino terminus of human TOR.
- L1210 cell lysate. ICC/IF: L1210 cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- DNA / RNA
- DNA Damage & Repair
- DNA Damage Response
- DNA Damage Recognition
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab25880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 289 kDa.Can be blocked with Human mTOR peptide (ab99155).|
FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Tissue specificityExpressed in numerous tissues, with highest levels in testis.
Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain.
modificationsAutophosphorylated; when part of mTORC1 or mTORC2.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
- Information by UniProt
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
Anti-mTOR antibody (ab25880) at 1 µg/ml + L1210 cell lysate
Predicted band size: 289 kDa
Observed band size: >204 kDa why is the actual band size different from the predicted?
ab25880 at 2µg/ml staining mTOR in L1210 cells by ICC/IF
ab25880 has been referenced in 3 publications.
- Wu SH et al. MiR-99a inhibits cell proliferation of nasopharyngeal carcinoma by targeting mTOR and serves as a prognostic factor. Eur Rev Med Pharmacol Sci 23:2053-2061 (2019). PubMed: 30915749
- Wang F et al. MiR-155-5p inhibits PDK1 and promotes autophagy via the mTOR pathway in cervical cancer. Int J Biochem Cell Biol 99:91-99 (2018). WB ; Human . PubMed: 29627439
- Zhang J et al. Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGK? gene knockout. Mol Med Rep 17:2169-2176 (2018). PubMed: 29207074