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    mtor-antibody-ab2732.pdf

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Anti-mTOR antibody (ab2732)

  • Datasheet
  • SDS
Reviews (14)Q&A (16)References (283)

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Western blot - Anti-mTOR antibody (ab2732)
  • Immunoprecipitation - Anti-mTOR antibody (ab2732)
  • Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)
  • Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)

Key features and details

  • Rabbit polyclonal to mTOR
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-mTOR antibody [Y391] (ab32028)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-mTOR antibody
    See all mTOR primary antibodies
  • Description

    Rabbit polyclonal to mTOR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human mTOR aa 200-250. The exact sequence is proprietary.
    Database link: P42345
    (Peptide available as ab39393)

  • Positive control

    • ICC/IF: HepG2 cells. L6 myotubes. IP: HeLa lysates. WB: HeLa, HEK-293T, Jurkat-HepG2 and LNCaP whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purification notes

    Affinity purified using the immunising peptideimmobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Metabolism
    • Types of disease
    • Obesity
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human mTOR protein (ab114179)
  • Related Products

    • Anti-mTOR antibody (ab2833)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/100.
IP
Use at 2-10 µg/mg of lysate.
WB (7)
1/2000 - 1/10000. Predicted molecular weight: 289 kDa.
Notes
ICC/IF
1/100.
IP
Use at 2-10 µg/mg of lysate.
WB
1/2000 - 1/10000. Predicted molecular weight: 289 kDa.

Target

  • Function

    Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificity

    Expressed in numerous tissues, with highest levels in testis.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications

    Autophosphorylated; when part of mTORC1 or mTORC2.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Target information above from: UniProt accession P42345 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2475 Human
    • Entrez Gene: 56717 Mouse
    • Entrez Gene: 56718 Rat
    • Omim: 601231 Human
    • SwissProt: P42345 Human
    • SwissProt: Q9JLN9 Mouse
    • SwissProt: P42346 Rat
    • Unigene: 338207 Human
    • Unigene: 21158 Mouse
    • Unigene: 11008 Rat
    see all
  • Alternative names

    • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
    • FK506 binding protein 12 rapamycin associated protein 1 antibody
    • FK506 binding protein 12 rapamycin associated protein 2 antibody
    • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
    • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
    • FKBP rapamycin associated protein antibody
    • FKBP12 rapamycin complex associated protein antibody
    • FKBP12-rapamycin complex-associated protein 1 antibody
    • FKBP12-rapamycin complex-associated protein antibody
    • FLJ44809 antibody
    • FRAP antibody
    • FRAP1 antibody
    • FRAP2 antibody
    • Mammalian target of rapamycin antibody
    • Mechanistic target of rapamycin antibody
    • mTOR antibody
    • MTOR_HUMAN antibody
    • OTTHUMP00000001983 antibody
    • RAFT1 antibody
    • Rapamycin and FKBP12 target 1 antibody
    • Rapamycin associated protein FRAP2 antibody
    • Rapamycin target protein 1 antibody
    • Rapamycin target protein antibody
    • RAPT1 antibody
    • Serine/threonine-protein kinase mTOR antibody
    see all

Images

  • Western blot - Anti-mTOR antibody (ab2732)
    Western blot - Anti-mTOR antibody (ab2732)
    All lanes : Anti-mTOR antibody (ab2732) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : LNCaP (Human prostate cancer cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 289 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN lysis buffer. 

  • Immunoprecipitation - Anti-mTOR antibody (ab2732)
    Immunoprecipitation - Anti-mTOR antibody (ab2732)

    mTOR was immunoprecipitated from HeLa cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab2732 at 3 µg per reaction. mTOR was also immunoprecipitated by ab2833. Western blot was performed from the immunoprecipitate with ab2732 at 1 µg/ml.

    Lane 1: ab2833 IP in HeLa whole cell lysate.
    Lane 2: ab2732 IP in HeLa whole cell lysate. 
    Lane 3: Control IgG IP in HeLa  whole cell lysate.

    Detection: Chemiluminescence

  • Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)
    Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)
    ICC/IF image of ab2732 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2732, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)
    Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)
    ab2732 at a 1:100 dilution confocally staining mTOR (red) in L6 myotubes, alongside a nuclear antigen antibody (green).    

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (283)

Publishing research using ab2732? Please let us know so that we can cite the reference in this datasheet.

ab2732 has been referenced in 283 publications.

  • Wang L  et al. LncRNA BCYRN1-induced autophagy enhances asparaginase resistance in extranodal NK/T-cell lymphoma. Theranostics 11:925-940 (2021). PubMed: 33391513
  • Chen MY  et al. Long non-coding RNA nuclear enriched abundant transcript 1 (NEAT1) sponges microRNA-124-3p to up-regulate phosphodiesterase 4B (PDE4B) to accelerate the progression of Parkinson's disease. Bioengineered 12:708-719 (2021). PubMed: 33522352
  • Tang J  et al. miR-27a promotes osteogenic differentiation in glucocorticoid-treated human bone marrow mesenchymal stem cells by targeting PI3K. J Mol Histol 52:279-288 (2021). PubMed: 33532936
  • Lv S  et al. QiShenYiQi pill activates autophagy to attenuate reactive myocardial fibrosis via the PI3K/AKT/mTOR pathway. Aging (Albany NY) 13:5525-5538 (2021). PubMed: 33582656
  • Liu Y  et al. GABARAP suppresses EMT and breast cancer progression via the AKT/mTOR signaling pathway. Aging (Albany NY) 13:5858-5874 (2021). PubMed: 33591943
View all Publications for this product

Customer reviews and Q&As

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1-10 of 14 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody

Average
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC2
Permeabilization
No
Specification
Liver
Blocking step
Dako Envision kit as blocking agent for 24 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 16 2021

Western blot abreview for Anti-mTOR antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (PC3 Prostate cancer cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
PC3 Prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

DR. Dimitra Kalamida

Verified customer

Submitted Jun 12 2019

Flow Cytometry abreview for Anti-mTOR antibody

Good
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (DU145 prostate cancer cell line)
Permeabilization
Yes - 0.1% Triton X-100
Gating Strategy
No gating was applied
Specification
DU145 prostate cancer cell line
Preparation
Cell harvesting/tissue preparation method: Cells were trypsinized
Sample buffer: PBS
Fixation
Paraformaldehyde
Read More

DR. Dimitra Kalamida

Verified customer

Submitted Nov 02 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-mTOR antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

DR. Dimitra Kalamida

Verified customer

Submitted Sep 07 2018

Immunohistochemistry free floating abreview for Anti-mTOR antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Read More

Abcam user community

Verified customer

Submitted Nov 11 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody

Good
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Equus Tissue sections (Lung)
Specification
Lung
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
Yes - 0.2% Triton-X100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 14 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric aicd
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Jul 06 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Specification
Testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More

MR. Carl Hobbs

Verified customer

Submitted Jul 06 2011

Western blot abreview for Anti-mTOR antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 cells)
Loading amount
10 µg
Specification
HT1080 cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Nov 18 2009

Western blot abreview for Anti-mTOR antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (L6 cell line)
Loading amount
10 µg
Specification
L6 cell line
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Nov 18 2009

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