Anti-mTOR antibody (ab2732)
- Datasheet
- References (104)
- Protocols
Overview
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Product nameAnti-mTOR antibody
See all mTOR primary antibodies -
DescriptionRabbit polyclonal to mTOR
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Fr, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human, Equus
Predicted to work with: Sheep, Rabbit, Goat, Guinea pig, Cow, Dog, Pig, Ferret, Rhesus monkey, Elephant
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Immunogen
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Positive control
- ICC/IF: HepG2 cells. L6 myotubes. IHC-P: Rat and mouse testis tissue. IP: HEK-293T lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purification notesAffinity purified using the immunising peptideimmobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/2000. | |
| IHC-Fr | 1/100. | |
| IP | 1/1000. | |
| IHC-P | 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
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Tissue specificityExpressed in numerous tissues, with highest levels in testis.
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Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
Post-translational
modificationsAutophosphorylated; when part of mTORC1 or mTORC2. -
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
- Information by UniProt
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Database links
- Entrez Gene: 2475 Human
- Entrez Gene: 56717 Mouse
- Entrez Gene: 56718 Rat
- Omim: 601231 Human
- SwissProt: P42345 Human
- SwissProt: Q9JLN9 Mouse
- SwissProt: P42346 Rat
- Unigene: 338207 Human
see all -
Alternative names
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)ICC/IF image of ab2732 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2732, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR antibody (ab2732)This image is courtesy of an Abreview submitted by Carl Hobbsab2732 staining rat testis sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab2732 at 1/2000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
Positive components in the submitted image are indicated by coloured arrowheads: red for densely positive nuclei that appear to be confined to the stem cell layer (immediately above, some spermatogonia exhibit a nuclear speckling). Yellow indicates a moderate cytoplasmic positivity, more evident in some seminiferous tubules (ST). Green for a strong apparently cytoplasmic positivity at the lumenal surface of a proportion of ST.
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Immunoprecipitation - Anti-mTOR antibody (ab2732)Detection of Human mTOR by Western Blot of Immunoprecipitates.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using lysis buffer.
Antibodies: Anti-mTOR antibody (ab2732) used for IP at 6 µg per reaction.
For blotting immunoprecipitated mTOR, ab2732 was used at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR antibody (ab2732)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdomab2732 staining mouse testis sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1%BSA for 10 minutes at 21°C, followed by staining with ab2732 at 1/3000 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
Positive components in the submitted image are indicated by coloured arrowheads: positive nuclei (red) appear to be confined to the stem cell layer of the seminiferous tubules whereas maturing cells appear to have light cytoplasmic positivity. In all tubes there is a lumenal cytoplasmic positivity that varies in intensity (green) between tubules. Leydig cells are also positive (yellow).
Spaces around the tubes are artefactual. -
Immunocytochemistry/ Immunofluorescence - Anti-mTOR antibody (ab2732)ab2732 at a 1:100 dilution confocally staining mTOR (red) in L6 myotubes, alongside a nuclear antigen antibody (green). -
Immunoprecipitation - Anti-mTOR antibody (ab2732)mTOR protein kinase assay:
ab2732 (1:1000 dilution) immunoprecipitates were incubated at 30°C with recombinant 4E-BP1 and 32P-gATP. Autoradiograph shows 32P incorporated into 4E-BP1.
Protocols
References
This product has been referenced in:
- Laurenzana A et al. EGFR/uPAR interaction as druggable target to overcome vemurafenib acquired resistance in melanoma cells. EBioMedicine 39:194-206 (2019). Read more (PubMed: 30611716) »
- Sun D et al. PUF60 accelerates the progression of breast cancer through downregulation of PTEN expression. Cancer Manag Res 11:821-830 (2019). Read more (PubMed: 30697074) »
