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I am attempting to immunoprecipitate mTOR and known interacting proteins (specifically Raptor and Rictor) with ab32028. I am successfully pulling down mTOR (as evidenced by Western blotting), but it is very inefficient and I am not seeing interacting proteins. Could I receive some advice/assistance on immunoprecipitation with this antibody? Please let me know what other info you would like from me about my protocol.
Asked on Aug 06 2012
Thank you for contacting Abcam.
I am sorry that you are having issues with ab32028 in IP. The antibody is covered under our Abpromise for six months and is guaranteed to work in IP on mouse, rat and human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.
To be able to help you futher, would you be able to send me some information on the protocol that you have been using with ab32028 eg how much antibody you are using, length of incubation, type of samples etc. Also, if possible, it would be very helpful if you could also supply an image of the results that you are getting with the antibody.
I look forward to your reply and helping to resolve this issue.
Answered on Aug 06 2012