• Product name

    Anti-mTOR (phospho S2448) antibody
    See all mTOR primary antibodies
  • Description

    Rabbit polyclonal to mTOR (phospho S2448)
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide: KRSRTRTDpSYSAGQSVEI, corresponding to C terminal amino acids 2440-2463 of Human mTOR.

  • Positive control

    • serum starved 293T cell lysate



Our Abpromise guarantee covers the use of ab1093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB 1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 288 kDa).
ELISA 1/6000 - 1/29000. Assayed aginst 0.1 µg of the immunizing peptide and has not been tested against the endogenous protein.


  • Function

    Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificity

    Expressed in numerous tissues, with highest levels in testis.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational

    Autophosphorylated; when part of mTORC1 or mTORC2.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Information by UniProt
  • Database links

  • Alternative names

    • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
    • FK506 binding protein 12 rapamycin associated protein 1 antibody
    • FK506 binding protein 12 rapamycin associated protein 2 antibody
    • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
    • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
    • FKBP rapamycin associated protein antibody
    • FKBP12 rapamycin complex associated protein antibody
    • FKBP12-rapamycin complex-associated protein 1 antibody
    • FKBP12-rapamycin complex-associated protein antibody
    • FLJ44809 antibody
    • FRAP antibody
    • FRAP1 antibody
    • FRAP2 antibody
    • Mammalian target of rapamycin antibody
    • Mechanistic target of rapamycin antibody
    • mTOR antibody
    • MTOR_HUMAN antibody
    • OTTHUMP00000001983 antibody
    • RAFT1 antibody
    • Rapamycin and FKBP12 target 1 antibody
    • Rapamycin associated protein FRAP2 antibody
    • Rapamycin target protein 1 antibody
    • Rapamycin target protein antibody
    • RAPT1 antibody
    • Serine/threonine-protein kinase mTOR antibody
    see all


  • Rabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa.
    Cells were serum starved for 24 hours:

    Lane 1 : control
    Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min

    Rabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa. Cells were serum starved for 24 hours:
    Lane 1 : control
    Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min

  • ab1093 at 5 µg/ml staining proximal convulted tubules of the kidney.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.


This product has been referenced in:

  • Teng CF  et al. Chemopreventive Effect of Phytosomal Curcumin on Hepatitis B Virus-Related Hepatocellular Carcinoma in A Transgenic Mouse Model. Sci Rep 9:10338 (2019). Read more (PubMed: 31316146) »
  • Ortega MA  et al. Implication of the PI3K/Akt/mTOR Pathway in the Process of Incompetent Valves in Patients with Chronic Venous Insufficiency and the Relationship with Aging. Oxid Med Cell Longev 2018:1495170 (2018). Read more (PubMed: 30057669) »
See all 13 Publications for this product

Customer reviews and Q&As


I'm sorry to hear one of your customers is experiencing a problem with ab1093. We have not tested this antibody in cultured HepG2 , HCT116, DLD1, SUV480 and HT29 cells and I think the customer's problem is due to the fact that these cells contain low levels of the phosphorylated protein, undetectable by WB with ab1093. For example, a paper by Paajarvi et al (FASEB J. 2005 Mar;19(3):476-8. Epub 2004 Dec 29.) shows that HepG2 cells (even serum starved) have very low levels of basal phosphoS448 mTOR and need to be stimulated with pravastin 2uM and insulin 1ugl/ml (1hr) to have a significant level of the phosphorylated protein. Therefore I strongly suggest that the customer runs the recommended positive control of serum starved 293T cell lysate (preferably nuclear lysate) and to also do a nuclear extraction step to concentrate the protein in her samples. Please ask her to try a 1:500 dilution of the antibody and make sure the secondary antibody is not damaged. I also noticed the customer blocked with two blocking agents (BSA and milk), this can cause problems too so ask her to do a 1hr 5%BSA block and use only TBST to dilute the primary and secondary antibodies (milk contains casein which is a phospho protein so causes interference with phospho-specific antibodies). Please let me know if you need any further assistance,

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