Western blot abreview for Anti-mTOR (phospho S2448) antibody - C-terminal

Inconclusive
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat primary cortical neuronal cultures)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
30 µg
Treatment
treated or not treated with rapamycin (500nM) 24h
Specification
Rat primary cortical neuronal cultures
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (casein 0.1%)
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit IgG from LiCOR (IRDye 800)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Odyssey Infrared Imaging System
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: >250kDa kDa Non-specific: ~130, ~90, ~40 kDa

Additional data

Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. As the product had not yet been tested in this species we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this species. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

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Submitted Sep 16 2019

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