RecombinantRabMAb

Recombinant Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)

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Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
  • Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR426(2)] to mTOR (phospho S2448) - BSA and Azide free
  • Suitable for: IHC-P, WB, Dot blot
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

HRP Unconjugated

Overview

  • Product name

    Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free
    See all mTOR primary antibodies

  • Description

    Rabbit monoclonal [EPR426(2)] to mTOR (phospho S2448) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab177734 is the carrier-free version of ab109268.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 289 kDa.
Dot blot
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 289 kDa.
Dot blot
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

    • Tissue specificity

      Expressed in numerous tissues, with highest levels in testis.

    • Sequence similarities

      Belongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 7 HEAT repeats.
      Contains 1 PI3K/PI4K domain.

    • Post-translational
      modifications

      Autophosphorylated; when part of mTORC1 or mTORC2.

    • Cellular localization

      Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
    • Information by UniProt

    • Database links

      • Alternative names

        • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
        • FK506 binding protein 12 rapamycin associated protein 1 antibody
        • FK506 binding protein 12 rapamycin associated protein 2 antibody
        • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
        • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
        • FKBP rapamycin associated protein antibody
        • FKBP12 rapamycin complex associated protein antibody
        • FKBP12-rapamycin complex-associated protein 1 antibody
        • FKBP12-rapamycin complex-associated protein antibody
        • FLJ44809 antibody
        • FRAP antibody
        • FRAP1 antibody
        • FRAP2 antibody
        • Mammalian target of rapamycin antibody
        • Mechanistic target of rapamycin antibody
        • mTOR antibody
        • MTOR_HUMAN antibody
        • OTTHUMP00000001983 antibody
        • RAFT1 antibody
        • Rapamycin and FKBP12 target 1 antibody
        • Rapamycin associated protein FRAP2 antibody
        • Rapamycin target protein 1 antibody
        • Rapamycin target protein antibody
        • RAPT1 antibody
        • Serine/threonine-protein kinase mTOR antibody
        see all

      Images

      • Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
        Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)

        Dot blot analysis of mTOR (phospho S2448) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2448) phospho peptide with purified ab109268 at a dilution of 1/1000 (0.073ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.

        Blocking buffer: 5% NFDM/TBST
        Diluting buffer: 5% NFDM /TBST

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109268).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)

        Immunohistochemical cytoplasmic and nuclear staining of paraffin embedded human endometrium carcinoma with purified ab109268 at a working dilution of 1 in 100. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109268).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)

        Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab109268 at a dilution of 1/50.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109268).

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
        Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)

      References (0)

      Publishing research using ab177734? Please let us know so that we can cite the reference in this datasheet.

      ab177734 has not yet been referenced specifically in any publications.

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