Recombinant
RabMAb

Recombinant Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)

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Overview

  • Product name

    Anti-mTOR (phospho S2481) antibody [EPR427(N)]
    See all mTOR primary antibodies

  • Description

    Rabbit monoclonal [EPR427(N)] to mTOR (phospho S2481)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human mTOR.
    (Peptide available as ab187360, ab187361)

  • Positive control

    • 293T and HeLa cell lysate

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 289 kDa.Can be blocked with mTOR (phospho S2481) peptide (ab187360) or mTOR peptide (ab187361).
Dot blot 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

    • Tissue specificity

      Expressed in numerous tissues, with highest levels in testis.

    • Sequence similarities

      Belongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 7 HEAT repeats.
      Contains 1 PI3K/PI4K domain.

    • Post-translational
      modifications

      Autophosphorylated; when part of mTORC1 or mTORC2.

    • Cellular localization

      Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
      • FK506 binding protein 12 rapamycin associated protein 1 antibody
      • FK506 binding protein 12 rapamycin associated protein 2 antibody
      • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
      • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
      • FKBP rapamycin associated protein antibody
      • FKBP12 rapamycin complex associated protein antibody
      • FKBP12-rapamycin complex-associated protein 1 antibody
      • FKBP12-rapamycin complex-associated protein antibody
      • FLJ44809 antibody
      • FRAP antibody
      • FRAP1 antibody
      • FRAP2 antibody
      • Mammalian target of rapamycin antibody
      • Mechanistic target of rapamycin antibody
      • mTOR antibody
      • MTOR_HUMAN antibody
      • OTTHUMP00000001983 antibody
      • RAFT1 antibody
      • Rapamycin and FKBP12 target 1 antibody
      • Rapamycin associated protein FRAP2 antibody
      • Rapamycin target protein 1 antibody
      • Rapamycin target protein antibody
      • RAPT1 antibody
      • Serine/threonine-protein kinase mTOR antibody
      see all

    Images

    • Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)
      Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)
      All lanes : Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate
      Lane 3 : HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 289 kDa
      Observed band size: 289 kDa



      Blocking Buffer: 5% NFDM/TBST
      Diluting Buffer: 5% NFDM/TBST

    • Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)
      Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)

      Dot blot analysis of mTOR (phospho S2481) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2481) phospho peptide with purified ab137133 at a dilution of 1/1000 (1.21ug/ml). A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

      Blocking buffer: 5% NFDM/TBST
      Diluting buffer: 5% NFDM /TBST

    • Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)
      Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)
      All lanes : Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution

      Lane 1 : 293 cell lysate, untreated
      Lane 2 : 293 cell lysate, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 289 kDa

    References

    This product has been referenced in:

    • Rui QH  et al. Effect of lncRNA HULC knockdown on rat secreting pituitary adenoma GH3 cells. Braz J Med Biol Res 52:e7728 (2019). Read more (PubMed: 30994730) »
    • Zhihua Y  et al. Hypoxia decreases macrophage glycolysis and M1 percentage by targeting microRNA-30c and mTOR in human gastric cancer. Cancer Sci 110:2368-2377 (2019). Read more (PubMed: 31222863) »
    See all 32 Publications for this product

    Publishing research using ab137133? Please let us know so that we can cite the reference in this datasheet.

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