Overview

  • Product name

  • Description

    Rabbit polyclonal to MTP18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Fusion protein corresponding to Human MTP18 (internal sequence). (BC046132. The identity of the protein fusion partner is GST).
    Database link: Q9UDX5

  • Positive control

    • Mouse heart, muscle, liver and kidney tissue lysates; HeLa and HepG2 whole cell lysate (ab7900); Human cervical cancer tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab198217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 18 kDa.

Target

  • Function

    Involved in the mitochondrial division probably by regulating membrane fission. Loss-of-function induces the release of cytochrome c, which activates the caspase cascade and leads to apoptosis.
  • Sequence similarities

    Belongs to the MTFP1 family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSPC 242 antibody
    • HSPC242 antibody
    • Mitochondrial 18 kDa protein antibody
    • Mitochondrial fission process protein 1 antibody
    • Mitochondrial fission protein MTP18 antibody
    • Mitochondrial protein 18 kDa antibody
    • MTFP1 antibody
    • MTFP1_HUMAN antibody
    • MTP 18 antibody
    • MTP18 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling MTP18 with ab198217 at 1/30 dilution.

  • All lanes : Anti-MTP18 antibody (ab198217) at 1/450 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse muscle tissue lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Mouse liver tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : HepG2 cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 18 kDa


    Exposure time: 5 seconds


    10% SDS-PAGE.

References

This product has been referenced in:

  • Wei Q  et al. MicroRNA-668 represses MTP18 to preserve mitochondrial dynamics in ischemic acute kidney injury. J Clin Invest 128:5448-5464 (2018). Read more (PubMed: 30325740) »
  • Aung LHH  et al. Mitochondrial protein 18 (MTP18) plays a pro-apoptotic role in chemotherapy-induced gastric cancer cell apoptosis. Oncotarget 8:56582-56597 (2017). WB . Read more (PubMed: 28915614) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Human gastric cancer cell)
Gel Running Conditions
Non-reduced Denaturing (Using 4-20% Tris Gel, running at 120 V for 1hr)
Loading amount
50 µg
Specification
Human gastric cancer cell
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Aug 06 2015

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