Key features and details
- Rabbit polyclonal to MTPAP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MTPAP antibody
See all MTPAP primary antibodies
DescriptionRabbit polyclonal to MTPAP
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human MTPAP aa 1-582.
Database link: Q9NVV4
- A549, HeLa, HepG2 and HCT116 cell lysates; HeLa cell
Previously labelled as Poly(A) RNA polymerase.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 66 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionPolymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
Tissue specificityUbiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle.
Involvement in diseaseDefects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).
Sequence similaritiesBelongs to the DNA polymerase type-B-like family.
Contains 1 PAP-associated domain.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- 0610027A18Rik antibody
- AW551379 antibody
- EC 188.8.131.52 antibody
ab154555 has been referenced in 1 publication.
- Toompuu M et al. Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells. Nucleic Acids Res 46:5209-5226 (2018). PubMed: 29518244