Key features and details
- Rabbit polyclonal to MTPAP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MTPAP antibody
See all MTPAP primary antibodies
DescriptionRabbit polyclonal to MTPAP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human MTPAP aa 532-582.
Database link: NP_060579.3
- HeLa, 293T and Jurkat whole cell lysates.
Previously labelled as Poly(A) RNA polymerase.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab156684 was affinity purified using an epitope specific to MTPAP, mitochondrial immobilized on solid support.
Our Abpromise guarantee covers the use of ab156684 in the following tested applications.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPolymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
Tissue specificityUbiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle.
Involvement in diseaseDefects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).
Sequence similaritiesBelongs to the DNA polymerase type-B-like family.
Contains 1 PAP-associated domain.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- 0610027A18Rik antibody
- AW551379 antibody
- EC 220.127.116.11 antibody
All lanes : Anti-MTPAP antibody (ab156684) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 30 seconds
Detection of MTPAP, mitochondrial by Western blot of Immunoprecipitate:
Immunoprecipitation of HeLa whole cell lysate using ab156684 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of MTPAP, mitochondrial with ab156684 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab156684 has not yet been referenced specifically in any publications.