Overview

  • Product name

  • Description

    Rabbit polyclonal to MTPAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human MTPAP aa 532-582.
    Database link: NP_060579.3

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.
  • General notes

    Previously labelled as Poly(A) RNA polymerase. 

Properties

Applications

Our Abpromise guarantee covers the use of ab156684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA.
  • Tissue specificity

    Ubiquitous, with stronger expression in tissues with high energy requirements: heart, brain, and skeletal muscle.
  • Involvement in disease

    Defects in MTPAP are the cause of spastic ataxia autosomal recessive type 4 (SPAX4) [MIM:613672]. A slowly progressive neurodegenerative disease characterized by cerebellar ataxia, spastic paraparesis, dysarthria, and optic atrophy. Note=Affected individuals exhibit a drastic decrease in poly(A) tail length of representative mitochondrial mRNA transcripts, including COX1 and RNA14 (PubMed:20970105).
  • Sequence similarities

    Belongs to the DNA polymerase type-B-like family.
    Contains 1 PAP-associated domain.
  • Cellular localization

    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610027A18Rik antibody
    • AW551379 antibody
    • EC 2.7.7.19 antibody
    • FLJ10486 antibody
    • mitochondrial antibody
    • Mitochondrial poly(A) polymerase antibody
    • mtPAP antibody
    • PAP antibody
    • PAP-associated domain-containing protein 1 antibody
    • PAPD1 antibody
    • PAPD1_HUMAN antibody
    • Poly(A) RNA polymerase antibody
    • Poly(A) RNA polymerase, mitochondrial antibody
    • Poly(A) RNA polymerase, mitochondrial precursor antibody
    • Polynucleotide adenylyltransferase antibody
    • RP11-305E6.3 antibody
    • Terminal uridylyltransferase 1 antibody
    • TUTase 1 antibody
    see all

Images

  • All lanes : Anti-MTPAP antibody (ab156684) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa


    Exposure time: 30 seconds
  • Detection of MTPAP, mitochondrial by Western blot of Immunoprecipitate:
    Immunoprecipitation of HeLa whole cell lysate using ab156684 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of MTPAP, mitochondrial with ab156684 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody.
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab156684 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab156684.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up