Overview

  • Product name

  • Description

    Rabbit polyclonal to MTR4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MTR4.
    Database link: NP_056175.2

  • Positive control

    • HeLa, and 293T whole cell lysates.
  • General notes

    Previously labelled as SKIV2L2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab70551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.

Epitope retrieval with Tris-EDTA pH9.0 is recommended for formalin-fixed paraffin-embedded cells.

WB 1/2000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      MTR4, or superkiller viralicidic activity 2 like 2, may be involved in pre-mRNA splicing. MTR4 is an exosome-associated protein and may be involved in the recruitment of the exosome to pre-rRNA, to mediate the 3' end processing of 5.8S rRNA.
    • Cellular localization

      Nucleus, nucleolus.
    • Database links

    • Alternative names

      • 2610528A15Rik antibody
      • ATP dependent helicase SKIV2L2 antibody
      • Dob1 antibody
      • KIAA0052 antibody
      • MGC142069 antibody
      • mKIAA0052 antibody
      • Mtr4 antibody
      • SKIV2L2 antibody
      • superkiller viralicidic activity 2 like 2 (S. cerevisiae) antibody
      • Superkiller viralicidic activity 2 like 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling MTR4 with ab70551 at 1/200 (1µg/ml). Detection: DAB.

    • All lanes : Anti-MTR4 antibody (ab70551) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Predicted band size: 118 kDa
      Observed band size: 118 kDa


      Exposure time: 10 seconds
    • IHC image of ab70551 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:

    • Bugai A  et al. P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress. Mol Cell 74:254-267.e10 (2019). Read more (PubMed: 30824372) »
    • Lingaraju M  et al. The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs. Nat Commun 10:3393 (2019). Read more (PubMed: 31358741) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100 µg
    Treatment
    siRNA validation
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Dec 06 2016

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 04 2013

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jan 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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