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    mtr4-antibody-ab70551.pdf

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Immunology Immune System Diseases Autoimmune
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Anti-MTR4 antibody (ab70551)

  • Datasheet
Reviews (3) Submit a question References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)
  • Western blot - Anti-MTR4 antibody (ab70551)
  • Immunoprecipitation - Anti-MTR4 antibody (ab70551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)

Key features and details

  • Rabbit polyclonal to MTR4
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MTR4 antibody
    See all MTR4 primary antibodies
  • Description

    Rabbit polyclonal to MTR4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MTR4.
    Database link: NP_056175.2

  • General notes

    Previously labelled as SKIV2L2. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab70551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

Target

  • Relevance

    MTR4, or superkiller viralicidic activity 2 like 2, may be involved in pre-mRNA splicing. MTR4 is an exosome-associated protein and may be involved in the recruitment of the exosome to pre-rRNA, to mediate the 3' end processing of 5.8S rRNA.
  • Cellular localization

    Nucleus, nucleolus.
  • Database links

    • Entrez Gene: 23517 Human
    • Entrez Gene: 72198 Mouse
    • SwissProt: P42285 Human
    • SwissProt: Q9CZU3 Mouse
    • Unigene: 274531 Human
    • Alternative names

      • 2610528A15Rik antibody
      • ATP dependent helicase SKIV2L2 antibody
      • Dob1 antibody
      • KIAA0052 antibody
      • MGC142069 antibody
      • mKIAA0052 antibody
      • Mtr4 antibody
      • SKIV2L2 antibody
      • superkiller viralicidic activity 2 like 2 (S. cerevisiae) antibody
      • Superkiller viralicidic activity 2 like 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling MTR4 with ab70551 at 1/200 (1µg/ml). Detection: DAB.

    • Western blot - Anti-MTR4 antibody (ab70551)
      Western blot - Anti-MTR4 antibody (ab70551)
      All lanes : Anti-MTR4 antibody (ab70551) at 0.1 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : HEK293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : TCMK-1 cell lysate
      Lane 5 : NIH3T3 cell lysate

      Predicted band size: 118 kDa



      Detection: Chemiluminescence with an exposure time of 3 minutes.

    • Immunoprecipitation - Anti-MTR4 antibody (ab70551)
      Immunoprecipitation - Anti-MTR4 antibody (ab70551)

      Detection of human MTR4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTR4 antibody ab70551 used for IP at 3 µg per reaction. MTR4 was also immunoprecipitated by rabbit anti-MTR4 antibody ab70552. For blotting immunoprecipitated MTR4, ab70551 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)
      IHC image of ab70551 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (5)

    Publishing research using ab70551? Please let us know so that we can cite the reference in this datasheet.

    ab70551 has been referenced in 5 publications.

    • Hou L  et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
    • Bugai A  et al. P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress. Mol Cell 74:254-267.e10 (2019). PubMed: 30824372
    • Lingaraju M  et al. The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs. Nat Commun 10:3393 (2019). PubMed: 31358741
    • Garland W  et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Rep 29:1800-1811.e6 (2019). PubMed: 31722198
    • Ebmeier CC & Taatjes DJ Activator-Mediator binding regulates Mediator-cofactor interactions. Proc Natl Acad Sci U S A 107:11283-8 (2010). WB ; Human . PubMed: 20534441

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews

    Western blot abreview for Anti-MTR4 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100 µg
    Treatment
    siRNA validation
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2016

    Western blot abreview for Anti-MTR4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 04 2013

    Western blot abreview for Anti-MTR4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 31 2012

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