Anti-MTR4 antibody (ab70551)
Key features and details
- Rabbit polyclonal to MTR4
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-MTR4 antibody
See all MTR4 primary antibodies -
Description
Rabbit polyclonal to MTR4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB MouseHuman
-
Immunogen
Synthetic peptide corresponding to Human MTR4.
Database link: NP_056175.2 -
General notes
Previously labelled as SKIV2L2.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Mouse
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB | (3) |
1/2000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). |
Target
-
Relevance
MTR4, or superkiller viralicidic activity 2 like 2, may be involved in pre-mRNA splicing. MTR4 is an exosome-associated protein and may be involved in the recruitment of the exosome to pre-rRNA, to mediate the 3' end processing of 5.8S rRNA. -
Cellular localization
Nucleus, nucleolus. -
Database links
- Entrez Gene: 23517 Human
- Entrez Gene: 72198 Mouse
- SwissProt: P42285 Human
- SwissProt: Q9CZU3 Mouse
- Unigene: 274531 Human
-
Alternative names
- 2610528A15Rik antibody
- ATP dependent helicase SKIV2L2 antibody
- Dob1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling MTR4 with ab70551 at 1/200 (1µg/ml). Detection: DAB.
-
Western blot - Anti-MTR4 antibody (ab70551)All lanes : Anti-MTR4 antibody (ab70551) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK-1 cell lysate
Lane 5 : NIH3T3 cell lysate
Predicted band size: 118 kDaDetection: Chemiluminescence with an exposure time of 3 minutes.
-
Immunoprecipitation - Anti-MTR4 antibody (ab70551)Detection of human MTR4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTR4 antibody ab70551 used for IP at 3 µg per reaction. MTR4 was also immunoprecipitated by rabbit anti-MTR4 antibody ab70552. For blotting immunoprecipitated MTR4, ab70551 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (ab70551)IHC image of ab70551 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab70551 has been referenced in 5 publications.
- Hou L et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
- Bugai A et al. P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress. Mol Cell 74:254-267.e10 (2019). PubMed: 30824372
- Lingaraju M et al. The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs. Nat Commun 10:3393 (2019). PubMed: 31358741
- Garland W et al. A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. Cell Rep 29:1800-1811.e6 (2019). PubMed: 31722198
- Ebmeier CC & Taatjes DJ Activator-Mediator binding regulates Mediator-cofactor interactions. Proc Natl Acad Sci U S A 107:11283-8 (2010). WB ; Human . PubMed: 20534441