Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%BSA in TBST
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: < 130 kDa
Additional data
Additional Notes
Samples in Laemmli buffer, standard Nitrocellulose membrane used.
Lane 1: U2OS whole cell extract, lane 2: U2OS whole cell extract transfected with GFP-SKIV2L2.
Abcam user community
Verified customer
Submitted Jan 31 2012