Western blot abreview for Anti-MTR4 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100 µg
Treatment
siRNA validation
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST, 0.05% Tween
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
4 unique siRNA sequences against SKIV2L2.
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 118 kDa Non-specific: 110, 54.53, 46, 45, 35 kDa
Positive control
siControl.

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Submitted Dec 06 2016

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