Key features and details
- Rabbit polyclonal to MTR4
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MTR4 antibody
See all MTR4 primary antibodies
DescriptionRabbit polyclonal to MTR4
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide corresponding to Human MTR4 aa 650-700.
Database link: NP_056175.2
- HeLa whole cell lysate (ab150035).
Previously labelled as SKIV2L2.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMTR4, or superkiller viralicidic activity 2 like 2, may be involved in pre-mRNA splicing. MTR4 is an exosome-associated protein and may be involved in the recruitment of the exosome to pre-rRNA, to mediate the 3' end processing of 5.8S rRNA.
Cellular localizationNucleus, nucleolus.
- 2610528A15Rik antibody
- ATP dependent helicase SKIV2L2 antibody
- Dob1 antibody
Detection of MTR4 by western blot of immunoprecipitates.
Samples: Lane 1: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer. Lane 2: Control IgG.
Antibodies: ab70552 used for IP at 6 µg per reaction. For blotting immunoprecipitated MTR4, ab70552 was used at 1 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab70552 has been referenced in 3 publications.
- Domanski M & LaCava J Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells. Bio Protoc 7:N/A (2017). PubMed: 28691041
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). PubMed: 28984244
- Onderak AM & Anderson JT Loss of the RNA helicase SKIV2L2 impairs mitotic progression and replication-dependent histone mRNA turnover in murine cell lines. RNA 23:910-926 (2017). PubMed: 28351885