Overview

  • Product name
    MTS Assay Kit (Cell Proliferation) (Colorimetric)
    See all Cell viability/proliferation kits
  • Detection method
    Colorimetric
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Assay time
    4h 00m
  • Product overview

    MTS Assay Kit ab197010 uses a colorimetric method for the sensitive quantification of viable cells. It can be used to assess cell proliferation, cell viability and cytotoxicity.


    The MTS assay protocol is based on the reduction of the MTS tetrazolium compound by viable mammalian cells (and cells from other species) to generate a colored formazan dye that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye is quantified by measuring the absorbance at 490-500 nm.


    The MTS assay protocol is performed by adding the MTS reagent directly into the cell culture media, without the intermittent steps which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in a 96-well microtiter plate.


    MTS assays are often used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. They are also used for the analysis of cytotoxic compounds like anticancer drugs and other toxic agents.

  • Notes
  • Platform
    Microplate reader

Properties

Associated products

Images

  • Jurkat cells were cultured at different densities overnight at 37°C in a final volume of 200 µL/well. MTS reagent was added and absorbance at OD=490 nm was recorded using ELISA plate reader. Each point represents a mean of 3 replicates. Assay was performed according to the kit protocol.

Protocols

References

This product has been referenced in:
  • Leipnitz G  et al. Evaluation of mitochondrial bioenergetics, dynamics, endoplasmic reticulum-mitochondria crosstalk, and reactive oxygen species in fibroblasts from patients with complex I deficiency. Sci Rep 8:1165 (2018). Human . Read more (PubMed: 29348607) »
  • Devarajan A  et al. Paraoxonase 2 overexpression inhibits tumor development in a mouse model of ovarian cancer. Cell Death Dis 9:392 (2018). Read more (PubMed: 29531225) »
See all 17 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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