Product nameMTT Assay Kit (Cell Proliferation)
See all Cell viability/proliferation kits
Sample typeAdherent cells, Suspension cells
Assay time3h 15m
Species reactivityReacts with: Other species, Mammals
MTT Assay Kit ab211091 provides a provides an easy-to-use, non-radioactive, and high-throughput method for measuring cell proliferation, cell viability and cytotoxicity.
The MTT assay protocol is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product.
Viable cells with active metabolism convert MTT into formazan. Dead cells, on the other hand, lose this ability and therefore show no signal. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance at OD 590 nm is proportional to the number of viable cells.
MTT assay protocol summary:
- replace serum-containing media with serum-free media and MTT reagent in cell cultures
- incubate for 3 hr at 37ºC
- add MTT solvent and incubate for 15 min
- analyze with microplate reader
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1000 tests MTT Reagent 1 x 50ml MTT Solvent 1 x 150ml
RelevanceCell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
HeLa cells were grown in DMEM media supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were then serially diluted in a clear cell culture plate and incubated for 3 hours with MTT Reagent at 37°C. After incubation, cells were treated with MTT Solvent for 15 minutes at room temperature. Absorbance was measured at OD = 590 nm. Inset graph is an expanded segment of the assay data at lower cell number per well.
This product has been referenced in:
- Cui Z et al. TRIM59 promotes gefitinib resistance in EGFR mutant lung adenocarcinoma cells. Life Sci 224:23-32 (2019). Read more (PubMed: 30902544) »
- De Felice F et al. Sulodexide counteracts endothelial dysfunction induced by metabolic or non-metabolic stresses through activation of the autophagic program. Eur Rev Med Pharmacol Sci 23:2669-2680 (2019). Read more (PubMed: 30964194) »