For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    mtt-assay-kit-cell-proliferation-ab211091.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Cell Metabolism Kits Other Metabolism Assay
Share by email

MTT Assay Kit (Cell Proliferation) (ab211091)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (41)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

MTT assay of Bortezomib in multiple myeloma cells
  • MTT Cell Proliferation Assay (ab211091)

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 3 hr 15 min
  • Sample type: Adherent cells, Suspension cells

You may also be interested in

ELISA
Product image
BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
ELISA
BrdU Cell Proliferation ELISA Kit (chemiluminescent) (ab126572)
Biochemical
Product image
Astaxanthin, Antioxidant agent (ab144193)

View more associated products

Overview

  • Product name

    MTT Assay Kit (Cell Proliferation)
    See all Cell viability/proliferation kits
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Assay time

    3h 15m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    MTT Assay Kit ab211091 is an easy-to-use, non-radioactive, and high-throughput assay for measuring cell proliferation, cell viability and cytotoxicity.


    The MTT assay protocol is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. NB: MTT is also available as free molecule as ab146345 (Thiazolyl blue tetrazolium bromide).


    Viable cells with active metabolism convert MTT into formazan. Dead cells lose this ability and therefore show no signal. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance at OD 590 nm is proportional to the number of viable cells.


    The MTT assay is a measure of the metabolic activity of the cells analyzed; the more metabolic activity in the sample, the higher the signal. This should be considered when interpreting the results of the assay. 


    MTT assay protocol summary:
    - replace serum-containing media with serum-free media and MTT reagent in cell cultures
    - incubate for 3 hr at 37ºC
    - add MTT solvent and incubate for 15 min
    - analyze with microplate reader

  • Notes

    Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay. An alternative product, MTS assay kit ab197010, uses a similar principle to this kit, but without the need for the MTT solvent step.

    How other researchers have used MTT Assay Kit ab211091

    This MTT assay kit has been used in publications with a variety of cell types, including:
    HUVEC cells1, U2OS cells and Saos2 cells2, human PBMCs3, rat primary hepatic stellate cells4, DBTRG glioblastoma cells5, SKOV3 human ovarian cancer cells6, mouse vascular smooth muscle cells7, human iPSC-derived neuronal cultures8, primary bladder cancer cells9 

    References: 1-De Felice F et al 2019, 2-Zhang X et al 2019, 3-Schotterl S et al 2018, 4-Huang W et al 2015, 5-Tang XJ et al 2016, 6-Zhu Q et al 2017, 7-Ma S et al 2018, 8-Rane A et al 2018, 9-Maj M et al 2018

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1000 tests
    MTT Reagent 1 x 50ml
    MTT Solvent 1 x 150ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Cell Viability and Senescence Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • proliferation and senescence
  • Relevance

    Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
  • Alternative names

    • Cell Cytotoxicity
    • cell tracking

Associated products

  • Related Products

    • Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye (ab146345)

Images

  • MTT assay of Bortezomib in multiple myeloma cells
    MTT assay of Bortezomib in multiple myeloma cellsImage courtesy of an anonymous abreview

    MTT assay kit ab211091 was used by an Abcam customer (see product review) to assess the cell viability of myeloma cells which have acquired resistance to bortezomib (a proteasome inhibitor used as a cancer therapeutic).

    Cells previously treated with 10 nM bortezomib for 1.5 months (RPMI8226-Bortezomib) had significantly improved viability, compared to the parental untreated cell line, when treated for 72 hours with varying concentrations of bortezomib.

  • MTT Cell Proliferation Assay (ab211091)
    MTT Cell Proliferation Assay (ab211091)

    HeLa cells were grown in DMEM media supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were then serially diluted in a clear cell culture plate and incubated for 3 hours with MTT Reagent at 37°C. After incubation, cells were treated with MTT Solvent for 15 minutes at room temperature. Absorbance was measured at OD = 590 nm. Inset graph is an expanded segment of the assay data at lower cell number per well.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (41)

Publishing research using ab211091? Please let us know so that we can cite the reference in this datasheet.

ab211091 has been referenced in 41 publications.

  • Wang J  et al. Bax inhibitor 1 preserves mitochondrial homeostasis in acute kidney injury through promoting mitochondrial retention of PHB2. Theranostics 10:384-397 (2020). PubMed: 31903127
  • Hawley SA  et al. Mechanism of Activation of AMPK by Cordycepin. Cell Chem Biol 27:214-222.e4 (2020). PubMed: 31991096
  • Yang L  et al. Therapeutic efficacy of catalpol against apoptosis in cardiomyocytes derived from human induced pluripotent stem cells. AMB Express 10:56 (2020). PubMed: 32198560
  • Mao Y  et al. The Overexpression of Epidermal Growth Factor (EGF) in HaCaT Cells Promotes the Proliferation, Migration, Invasion and Transdifferentiation to Epidermal Stem Cell Immunophenotyping of Adipose-Derived Stem Cells (ADSCs). Int J Stem Cells 13:93-103 (2020). PubMed: 32114740
  • Shi L  et al. Exosomal miRNA-34 from cancer-associated fibroblasts inhibits growth and invasion of gastric cancer cells in vitro and in vivo. Aging (Albany NY) 12:8549-8564 (2020). PubMed: 32391804
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

MTT assay of Bortezomib in multiple myeloma cells

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
1×105 cells exposed upon 10 nM bortezomib for 1.5 months in 100 µl media were seeded in each well of 96-well plates. The cells were then incubated with gradient dilutions of bortezomib at final concentrations of 0, 0.15625, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 nM 37°C in a total of 150 µl media in humidified incubator containing 5% CO2. After 72 hours of incubation, cell viability was examined by MTT assay. Experiments were carried out in quadruplicate, and the data were processed with GraphPad Prism. The results were shown as mean ± SD of three independent experiments.

Abcam user community

Verified customer

Submitted Jun 22 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.