MTT Assay Kit (Cell Proliferation) (ab211091)

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Overview

  • Product name

    MTT Assay Kit (Cell Proliferation)
    See all Cell viability/proliferation kits

  • Detection method

    Colorimetric

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Quantitative

  • Assay time

    3h 15m

  • Species reactivity

    Reacts with: Other species, Mammals

  • Product overview

    MTT Assay Kit ab211091 provides an easy-to-use, non-radioactive, and high-throughput method for measuring cell proliferation, cell viability and cytotoxicity.


    The MTT assay protocol is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product.


    Viable cells with active metabolism convert MTT into formazan. Dead cells, on the other hand, lose this ability and therefore show no signal. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance at OD 590 nm is proportional to the number of viable cells.


    MTT assay protocol summary:
    - replace serum-containing media with serum-free media and MTT reagent in cell cultures
    - incubate for 3 hr at 37ºC
    - add MTT solvent and incubate for 15 min
    - analyze with microplate reader

  • Notes

    Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay. An alternative product, MTS assay kit ab197010, uses a similar principle to this kit, but without the need for the MTT solvent step.

    How other researchers have used MTT Assay Kit ab211091

    This MTT assay kit has been used in publications with a variety of cell types, including:
    HUVEC cells1, U2OS cells and Saos2 cells2, human PBMCs3, rat primary hepatic stellate cells4, DBTRG glioblastoma cells5, SKOV3 human ovarian cancer cells6, mouse vascular smooth muscle cells7, human iPSC-derived neuronal cultures8, primary bladder cancer cells9 

    References: 1-De Felice F et al 2019, 2-Zhang X et al 2019, 3-Schotterl S et al 2018, 4-Huang W et al 2015, 5-Tang XJ et al 2016, 6-Zhu Q et al 2017, 7-Ma S et al 2018, 8-Rane A et al 2018, 9-Maj M et al 2018

  • Platform

    Microplate reader

Properties

Images

  • MTT assay of Bortezomib in multiple myeloma cells
    MTT assay of Bortezomib in multiple myeloma cellsImage courtesy of an anonymous abreview

    MTT assay kit ab211091 was used by an Abcam customer (see product review) to assess the cell viability of myeloma cells which have acquired resistance to bortezomib (a proteasome inhibitor used as a cancer therapeutic).

    Cells previously treated with 10 nM bortezomib for 1.5 months (RPMI8226-Bortezomib) had significantly improved viability, compared to the parental untreated cell line, when treated for 72 hours with varying concentrations of bortezomib.

  • MTT Cell Proliferation Assay (ab211091)
    MTT Cell Proliferation Assay (ab211091)

    HeLa cells were grown in DMEM media supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were then serially diluted in a clear cell culture plate and incubated for 3 hours with MTT Reagent at 37°C. After incubation, cells were treated with MTT Solvent for 15 minutes at room temperature. Absorbance was measured at OD = 590 nm. Inset graph is an expanded segment of the assay data at lower cell number per well.

Protocols

References

This product has been referenced in:

  • Cui Z  et al. TRIM59 promotes gefitinib resistance in EGFR mutant lung adenocarcinoma cells. Life Sci 224:23-32 (2019). Read more (PubMed: 30902544) »
  • De Felice F  et al. Sulodexide counteracts endothelial dysfunction induced by metabolic or non-metabolic stresses through activation of the autophagic program. Eur Rev Med Pharmacol Sci 23:2669-2680 (2019). Read more (PubMed: 30964194) »
See all 20 Publications for this product

Publishing research using ab211091? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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MTT assay of Bortezomib in multiple myeloma cells

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Abreviews
abreview image
1×105 cells exposed upon 10 nM bortezomib for 1.5 months in 100 µl media were seeded in each well of 96-well plates. The cells were then incubated with gradient dilutions of bortezomib at final concentrations of 0, 0.15625, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 nM 37°C in a total of 150 µl media in humidified incubator containing 5% CO2. After 72 hours of incubation, cell viability was examined by MTT assay. Experiments were carried out in quadruplicate, and the data were processed with GraphPad Prism. The results were shown as mean ± SD of three independent experiments.

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Submitted Jun 22 2018

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