• Product name
    MTT Assay Kit (Cell Proliferation)
    See all Cell viability/proliferation kits
  • Detection method
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
  • Assay time
    3h 15m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    MTT Assay Kit ab211091 provides a provides an easy-to-use, non-radioactive, and high-throughput method for measuring cell proliferation, cell viability and cytotoxicity.

    The MTT assay protocol is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product.

    Viable cells with active metabolism convert MTT into formazan. Dead cells, on the other hand, lose this ability and therefore show no signal. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance at OD 590 nm is proportional to the number of viable cells.

    MTT assay protocol summary:
    - replace serum-containing media with serum-free media and MTT reagent in cell cultures
    - incubate for 3 hr at 37ºC
    - add MTT solvent and incubate for 15 min
    - analyze with microplate reader

  • Notes

    Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.

  • Platform
    Microplate reader



  • HeLa cells were grown in DMEM media supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were then serially diluted in a clear cell culture plate and incubated for 3 hours with MTT Reagent at 37°C. After incubation, cells were treated with MTT Solvent for 15 minutes at room temperature. Absorbance was measured at OD = 590 nm. Inset graph is an expanded segment of the assay data at lower cell number per well.



This product has been referenced in:
  • Cui Z  et al. TRIM59 promotes gefitinib resistance in EGFR mutant lung adenocarcinoma cells. Life Sci 224:23-32 (2019). Read more (PubMed: 30902544) »
  • De Felice F  et al. Sulodexide counteracts endothelial dysfunction induced by metabolic or non-metabolic stresses through activation of the autophagic program. Eur Rev Med Pharmacol Sci 23:2669-2680 (2019). Read more (PubMed: 30964194) »
See all 7 Publications for this product

Customer reviews and Q&As

MTT assay of Bortezomib in multiple myeloma cells

Excellent Excellent 5/5 (Ease of Use)
1×105 cells exposed upon 10 nM bortezomib for 1.5 months in 100 µl media were seeded in each well of 96-well plates. The cells were then incubated with gradient dilutions of bortezomib at final concentrations of 0, 0.15625, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 nM 37°C in a total of 150 µl media in humidified incubator containing 5% CO2. After 72 hours of incubation, cell viability was examined by MTT assay. Experiments were carried out in quadruplicate, and the data were processed with GraphPad Prism. The results were shown as mean ± SD of three independent experiments.

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Submitted Jun 22 2018

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