Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684)


  • Product name
    Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker
    See all mtTFA primary antibodies
  • Description
    Mouse monoclonal [18G102B2E11] to mtTFA - Mitochondrial Marker
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, In-Cell ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Human, full-length (minus the N-terminal mitochondrial targeting sequence)

  • Positive control
    • IHC-P: human liver FFPE tissue sections
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab119684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.


  • Function
    Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
  • Sequence similarities
    Contains 2 HMG box DNA-binding domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • anscription factor 6-like 1 antibody
    • Mitochondrial transcription factor 1 antibody
    • mitochondrial transcription factor A antibody
    • MtTF1 antibody
    • mtTFA antibody
    • TCF 6 antibody
    • TCF-6 antibody
    • TCF6 antibody
    • TCF6L1 antibody
    • TCF6L2 antibody
    • TCF6L3 antibody
    • TFAM antibody
    • TFAM_HUMAN antibody
    • Transcription factor 6 antibody
    • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
    • Transcription factor 6 like 2 antibody
    • Transcription factor 6-like 2 antibody
    • transcription factor 6-like 3 antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial precursor antibody
    see all


  • Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.
  • HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • All lanes : Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684) at 1 µg/ml

    Lane 1 : recombinant mtTFA (ab103784) at 0.025 µg
    Lane 2 : HeLa whole cell lysate at 30 µg

    All lanes : HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution

    Predicted band size: 29 kDa

  • IHC image of mtTFA staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119684, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Peter B  et al. Defective mitochondrial protease LonP1 can cause classical mitochondrial disease. Hum Mol Genet 27:1743-1753 (2018). Read more (PubMed: 29518248) »
  • Špacek T  et al. Nkx6.1 decline accompanies mitochondrial DNA reduction but subtle nucleoid size decrease in pancreatic islet ß-cells of diabetic Goto Kakizaki rats. Sci Rep 7:15674 (2017). Read more (PubMed: 29142323) »

See all 11 Publications for this product

Customer reviews and Q&As

Western blot
Human Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 10 2015

Upon reviewing your protocol, it is troubling to me that all 4 of these antibodies are not working. While it is conceivable that all four antibodies are not performing properly, typically when multiple antibodies are not working it is an issue wit...

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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée que vous avez des problèmes avec l'interprétation de deux de nos anticorps.

Comme expliqué aupara...

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Je suis désolée d'apprendre que ces anticorps ne vous donnent pas de résultats satisfaisants en Western blot - c.à.d. une bande à environ 42kD pour SURF1 et à environ 24kD pour mtTFA).
Nous n'avons pas eu des autres retours négatives et n'avons pa...

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