Overview

  • Product name

    Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker
    See all mtTFA primary antibodies
  • Description

    Mouse monoclonal [18G102B2E11] to mtTFA - Mitochondrial Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, In-Cell ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: human liver FFPE tissue sections
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab119684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
  • Sequence similarities

    Contains 2 HMG box DNA-binding domains.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • anscription factor 6-like 1 antibody
    • Mitochondrial transcription factor 1 antibody
    • mitochondrial transcription factor A antibody
    • MtTF1 antibody
    • mtTFA antibody
    • TCF 6 antibody
    • TCF-6 antibody
    • TCF6 antibody
    • TCF6L1 antibody
    • TCF6L2 antibody
    • TCF6L3 antibody
    • TFAM antibody
    • TFAM_HUMAN antibody
    • Transcription factor 6 antibody
    • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
    • Transcription factor 6 like 2 antibody
    • Transcription factor 6-like 2 antibody
    • transcription factor 6-like 3 antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial precursor antibody
    see all

Images

  • All lanes : Anti-mtTFA antibody [18G102B2E11] - Mitochondrial Marker (ab119684) at 1 µg/ml

    Lane 1 : recombinant mtTFA (ab103784) at 0.025 µg
    Lane 2 : HeLa whole cell lysate at 30 µg

    Secondary
    All lanes : HRP-conjugated Goat polyclonal to mouse IgG at 1/5000 dilution

    Predicted band size: 29 kDa

  • Immunocytochemistry image of ab119684 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (18G102B2E11, 1µg/ml) for 2h at room temperature. The secondary antibody was (green) 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). mtTFA localizes to the mitochondria.
  • HeLa cells were stained with 2 µg/mL mtTFA antibody (ab119684) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • IHC image of mtTFA staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119684, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Li Q  et al. Ochratoxin A causes mitochondrial dysfunction, apoptotic and autophagic cell death and also induces mitochondrial biogenesis in human gastric epithelium cells. Arch Toxicol 93:1141-1155 (2019). Read more (PubMed: 30903243) »
  • Li H  et al. Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations. Autophagy 15:113-130 (2019). Read more (PubMed: 30160596) »
See all 17 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Human Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 10 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up