Western blot abreview for Anti-mtTFA antibody [18G102B2E11]

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name

Detection

Detection method
Chemi west Pico
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 23 kDa

Additional data

Additional Notes
Ladder (SMOBio P5200) image taken at same time as TFAM image, without moving the blots. Lane 1 = Aged Male subject, Lane 2 = Aged Female Subject

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Submitted Jul 10 2015

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