Key features and details
- Rabbit polyclonal to mtTFA
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-mtTFA antibody
See all mtTFA primary antibodies
DescriptionRabbit polyclonal to mtTFA
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide, corresponding to a region between amino acids 196-246 of Human mtTFA (NP_003192.1).
- Hela, 293T and Jurkat whole cell lysates
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab114993 was affinity purified using an epitope specific to mtTFA immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Our Abpromise guarantee covers the use of ab114993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 29 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
Sequence similaritiesContains 2 HMG box DNA-binding domains.
- Information by UniProt
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
All lanes : Anti-mtTFA antibody (ab114993) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 30 seconds
Lane 1: ab114993, at 1 µg/ml, staining mtTFA in HeLa whole cell lysate immunoprecipitated using ab114993 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab114993 has not yet been referenced specifically in any publications.