Anti-mtTFA antibody (ab155240)
Key features and details
- Rabbit polyclonal to mtTFA
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-mtTFA antibody
See all mtTFA primary antibodies -
Description
Rabbit polyclonal to mtTFA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-246 of Human mtTFA (Q00059)
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Positive control
- WB: HEK-293T, A431, HeLa and HepG2 cell lysate; IHC-P: Human colon and esophagus cancer tissue; IF: HeLa whole cells; IP: HEK-293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
|
| IHC-P |
1/100 - 1/1000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
|
IHC-P
1/100 - 1/1000. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7019 Human
- Omim: 600438 Human
- SwissProt: Q00059 Human
- Unigene: 594250 Human
- Unigene: 642966 Human
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Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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Western blot - Anti-mtTFA antibody (ab155240)All lanes : Anti-mtTFA antibody (ab155240) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 29 kDa12% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)Immunohistochemical analysis of human colon cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab155240)Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling mtTFA protein at mitochondria (green) with ab155240 at 1/1000 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/10000 dilution. Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-mtTFA antibody (ab155240)IP Sample:
1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, 40 µg;
2: Control with 2 μg of preimmune rabbit IgG;
3: Immunoprecipitation of mtTFA protein by 2 μg of ab155240.
The immunoprecipitated mtTFA protein was detected by ab155240 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)Immunohistochemical analysis of human esophagus cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Protocols
References (2)
ab155240 has been referenced in 2 publications.
- Lia D et al. Mitochondrial maintenance under oxidative stress depends on mitochondrially localised a-OGG1. J Cell Sci 131:N/A (2018). PubMed: 29848661
- Vishnyakova PA et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). PubMed: 28736722
