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    mttfa-antibody-ab155240.pdf

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Anti-mtTFA antibody (ab155240)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Anti-mtTFA antibody (ab155240)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)
  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab155240)
  • Immunoprecipitation - Anti-mtTFA antibody (ab155240)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)

Key features and details

  • Rabbit polyclonal to mtTFA
  • Suitable for: IP, WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human mtTFA protein (ab103784)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-mtTFA antibody
    See all mtTFA primary antibodies
  • Description

    Rabbit polyclonal to mtTFA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-246 of Human mtTFA (Q00059)

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 cell lysate; IHC-P: Human colon and esophagus cancer tissue; IF: HeLa whole cells; IP: HEK-293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human mtTFA protein (ab103784)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
IP
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
  • Sequence similarities

    Contains 2 HMG box DNA-binding domains.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q00059 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7019 Human
    • Omim: 600438 Human
    • SwissProt: Q00059 Human
    • Unigene: 594250 Human
    • Unigene: 642966 Human
    • Alternative names

      • anscription factor 6-like 1 antibody
      • Mitochondrial transcription factor 1 antibody
      • mitochondrial transcription factor A antibody
      • MtTF1 antibody
      • mtTFA antibody
      • TCF 6 antibody
      • TCF-6 antibody
      • TCF6 antibody
      • TCF6L1 antibody
      • TCF6L2 antibody
      • TCF6L3 antibody
      • TFAM antibody
      • TFAM_HUMAN antibody
      • Transcription factor 6 antibody
      • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
      • Transcription factor 6 like 2 antibody
      • Transcription factor 6-like 2 antibody
      • transcription factor 6-like 3 antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial precursor antibody
      see all

    Images

    • Western blot - Anti-mtTFA antibody (ab155240)
      Western blot - Anti-mtTFA antibody (ab155240)
      All lanes : Anti-mtTFA antibody (ab155240) at 1/5000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 29 kDa



      12% SDS PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)

      Immunohistochemical analysis of human colon cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

    • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab155240)
      Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab155240)

      Immunofluorescence analysis of 4% paraformaldehyde-fixed (RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling mtTFA protein at mitochondria (green) with ab155240 at 1/1000 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/10000 dilution. Blue: Hoechst 33342 staining.

    • Immunoprecipitation - Anti-mtTFA antibody (ab155240)
      Immunoprecipitation - Anti-mtTFA antibody (ab155240)

      IP Sample:

      1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate, 40 µg;

      2: Control with 2 μg of preimmune rabbit IgG;

      3: Immunoprecipitation of mtTFA protein by 2 μg of ab155240.

      The immunoprecipitated mtTFA protein was detected by ab155240 at 1/1000 dilution, followed by anti-rabbit IgG antibody.

      15% SDS-PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab155240)

      Immunohistochemical analysis of human esophagus cancer tissue staining mtTFA protein at cytoplasm with ab155240 at 1/500 dilution.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab155240? Please let us know so that we can cite the reference in this datasheet.

    ab155240 has been referenced in 2 publications.

    • Lia D  et al. Mitochondrial maintenance under oxidative stress depends on mitochondrially localised a-OGG1. J Cell Sci 131:N/A (2018). PubMed: 29848661
    • Vishnyakova PA  et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). PubMed: 28736722

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-mtTFA antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Placenta)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    50 µg
    Specification
    Placenta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Dr. Polina Vishnyakova

    Verified customer

    Submitted Apr 01 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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