Recombinant
RabMAb

Anti-mtTFA antibody [EPR12286] (ab171951)

Overview

  • Product name
    Anti-mtTFA antibody [EPR12286]
    See all mtTFA primary antibodies
  • Description
    Rabbit monoclonal [EPR12286] to mtTFA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q00059

  • Positive control
    • A431, HeLa, MCF-7, K562 and 293T cell lysates. Paraffin-embedded human ovarian carcinoma tissue and human testis tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
    • Sequence similarities
      Contains 2 HMG box DNA-binding domains.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • anscription factor 6-like 1 antibody
      • Mitochondrial transcription factor 1 antibody
      • mitochondrial transcription factor A antibody
      • MtTF1 antibody
      • mtTFA antibody
      • TCF 6 antibody
      • TCF-6 antibody
      • TCF6 antibody
      • TCF6L1 antibody
      • TCF6L2 antibody
      • TCF6L3 antibody
      • TFAM antibody
      • TFAM_HUMAN antibody
      • Transcription factor 6 antibody
      • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
      • Transcription factor 6 like 2 antibody
      • Transcription factor 6-like 2 antibody
      • transcription factor 6-like 3 antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial precursor antibody
      see all

    Images

    • ab171951 staining mtTFA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling mtTFA with ab171951 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue  labeling mtTFA with ab171951 at 1/100 dilution.

    • All lanes : Anti-mtTFA antibody [EPR12286] (ab171951)

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    References

    ab171951 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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