• Product name

    Anti-mtTFA antibody [EPR12286]
    See all mtTFA primary antibodies
  • Description

    Rabbit monoclonal [EPR12286] to mtTFA
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q00059

  • Positive control

    • A431, HeLa, MCF-7, K562 and 293T cell lysates. Paraffin-embedded human ovarian carcinoma tissue and human testis tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab171951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
    • Sequence similarities

      Contains 2 HMG box DNA-binding domains.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • anscription factor 6-like 1 antibody
      • Mitochondrial transcription factor 1 antibody
      • mitochondrial transcription factor A antibody
      • MtTF1 antibody
      • mtTFA antibody
      • TCF 6 antibody
      • TCF-6 antibody
      • TCF6 antibody
      • TCF6L1 antibody
      • TCF6L2 antibody
      • TCF6L3 antibody
      • TFAM antibody
      • TFAM_HUMAN antibody
      • Transcription factor 6 antibody
      • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
      • Transcription factor 6 like 2 antibody
      • Transcription factor 6-like 2 antibody
      • transcription factor 6-like 3 antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial antibody
      • Transcription factor A, mitochondrial precursor antibody
      see all


    • ab171951 staining mtTFA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling mtTFA with ab171951 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue  labeling mtTFA with ab171951 at 1/100 dilution.

    • All lanes : Anti-mtTFA antibody [EPR12286] (ab171951)

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa


    ab171951 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 29 2016

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