Product nameAnti-mtTFA antibody [EPR12286]
See all mtTFA primary antibodies
DescriptionRabbit monoclonal [EPR12286] to mtTFA
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q00059
- A431, HeLa, MCF-7, K562 and 293T cell lysates. Paraffin-embedded human ovarian carcinoma tissue and human testis tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Our Abpromise guarantee covers the use of ab171951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
Sequence similaritiesContains 2 HMG box DNA-binding domains.
- Information by UniProt
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
ab171951 staining mtTFA in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling mtTFA with ab171951 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling mtTFA with ab171951 at 1/100 dilution.
All lanes : Anti-mtTFA antibody [EPR12286] (ab171951)
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
ab171951 has not yet been referenced specifically in any publications.