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    mttfa-antibody-mitochondrial-marker-ab131607.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-mtTFA antibody - Mitochondrial Marker (ab131607)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (79)

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Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)

Key features and details

  • Rabbit polyclonal to mtTFA - Mitochondrial Marker
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-mtTFA antibody [EPR12285] (ab176558)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-mtTFA antibody - Mitochondrial Marker
    See all mtTFA primary antibodies
  • Description

    Rabbit polyclonal to mtTFA - Mitochondrial Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Mouse mtTFA. Native recombinant mouse mtTFA protein with a C-terminal 6-His tag.

  • Positive control

    • Rat kidney lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab131607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 28 kDa.

Target

  • Function

    Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.
  • Sequence similarities

    Contains 2 HMG box DNA-binding domains.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q00059 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7019 Human
    • Entrez Gene: 21780 Mouse
    • Entrez Gene: 83474 Rat
    • Omim: 600438 Human
    • SwissProt: Q00059 Human
    • SwissProt: P40630 Mouse
    • SwissProt: Q91ZW1 Rat
    • Unigene: 594250 Human
    • Unigene: 642966 Human
    • Unigene: 229292 Mouse
    • Unigene: 18266 Rat
    see all
  • Alternative names

    • anscription factor 6-like 1 antibody
    • Mitochondrial transcription factor 1 antibody
    • mitochondrial transcription factor A antibody
    • MtTF1 antibody
    • mtTFA antibody
    • TCF 6 antibody
    • TCF-6 antibody
    • TCF6 antibody
    • TCF6L1 antibody
    • TCF6L2 antibody
    • TCF6L3 antibody
    • TFAM antibody
    • TFAM_HUMAN antibody
    • Transcription factor 6 antibody
    • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
    • Transcription factor 6 like 2 antibody
    • Transcription factor 6-like 2 antibody
    • transcription factor 6-like 3 antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial precursor antibody
    see all

Images

  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
    Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
    All lanes : Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution

    Lane 1 : Mouse liver whole tissue lysate
    Lane 2 : Mouse hepatocytes whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa


    Exposure time: 10 seconds

    See Abreview

  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
    Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
    Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution + Rat kidney lysate

    Predicted band size: 28 kDa

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (79)

    Publishing research using ab131607? Please let us know so that we can cite the reference in this datasheet.

    ab131607 has been referenced in 79 publications.

    • Esteves P  et al. Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F. Oncogene 39:617-636 (2020). PubMed: 31527668
    • Kang JG  et al. A Mouse Homolog of a Human TP53 Germline Mutation Reveals a Lipolytic Activity of p53. Cell Rep 30:783-792.e5 (2020). PubMed: 31968253
    • Feng LR  et al. Cancer-related fatigue during combined treatment of androgen deprivation therapy and radiotherapy is associated with mitochondrial dysfunction. Int J Mol Med 45:485-496 (2020). PubMed: 31894256
    • Tang X  et al. Evaluating the Activity of Sodium Butyrate to Prevent Osteoporosis in Rats by Promoting Osteal GSK-3ß/Nrf2 Signaling and Mitochondrial Function. J Agric Food Chem 68:6588-6603 (2020). PubMed: 32459091
    • Bi J  et al. Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver Injury. Oxid Med Cell Longev 2020:6946037 (2020). PubMed: 31976032
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-mtTFA antibody - Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (retina)
    Permeabilization
    Yes - triton x-100
    Specification
    retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 10 2019

    Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEFs)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    MEFs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 03 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-mtTFA antibody - Mitochondrial Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 14 2017

    Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Soleus muscle)
    Gel Running Conditions
    Reduced Denaturing (4-12% NuPAGE gel)
    Loading amount
    25 µg
    Specification
    Soleus muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Ms. Jenny Sharpe

    Verified customer

    Submitted Jun 29 2015

    Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (liver, hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    liver, hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 14 2015

    Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela, Hep G2)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Hela, Hep G2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 06 2015

    Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cynomolgus monkey Tissue lysate - whole (Peripheral Blood Mononuclear Cell)
    Gel Running Conditions
    Reduced Denaturing (12% Bis-Tris)
    Loading amount
    20 µg
    Specification
    Peripheral Blood Mononuclear Cell
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Mr. Daniel Tyrrell

    Verified customer

    Submitted Sep 30 2013

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