Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
Key features and details
- Rabbit polyclonal to mtTFA - Mitochondrial Marker
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-mtTFA antibody - Mitochondrial Marker
See all mtTFA primary antibodies -
Description
Rabbit polyclonal to mtTFA - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length protein (His-tag) corresponding to Mouse mtTFA. Native recombinant mouse mtTFA protein with a C-terminal 6-His tag.
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Positive control
- Rat kidney lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
1/2000. Predicted molecular weight: 28 kDa.
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| Notes |
|---|
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WB
1/2000. Predicted molecular weight: 28 kDa. |
Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 83474 Rat
- SwissProt: Q91ZW1 Rat
- Unigene: 18266 Rat
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Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)All lanes : Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution
Lane 1 : Mouse liver whole tissue lysate
Lane 2 : Mouse hepatocytes whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Exposure time: 10 seconds
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Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution + Rat kidney lysate
Predicted band size: 28 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (109)
ab131607 has been referenced in 109 publications.
- Zhang G et al. Renovascular effects of inorganic nitrate following ischemia-reperfusion of the kidney. Redox Biol 39:101836 (2021). PubMed: 33360353
- Ghazal N et al. Mitochondrial functional resilience after TFAM ablation in the adult heart. Am J Physiol Cell Physiol 320:C929-C942 (2021). PubMed: 33760663
- Hu J et al. Elevated Lactate by High-Intensity Interval Training Regulates the Hippocampal BDNF Expression and the Mitochondrial Quality Control System. Front Physiol 12:629914 (2021). PubMed: 33716776
- Hu M et al. ATM inhibition enhances cancer immunotherapy by promoting mtDNA leakage and cGAS/STING activation. J Clin Invest 131:N/A (2021). PubMed: 33290271
- Tu T et al. Irisin Contributes to Neuroprotection by Promoting Mitochondrial Biogenesis After Experimental Subarachnoid Hemorrhage. Front Aging Neurosci 13:640215 (2021). PubMed: 33613273