Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
Rabbit polyclonal mtTFA antibody. Validated in WB and tested in Mouse, Rat, Human. Cited in 56 publication(s). Independently reviewed in 9 review(s).
- Datasheet
- References (62)
- Protocols
Overview
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Product name
Anti-mtTFA antibody - Mitochondrial Marker
See all mtTFA primary antibodies -
Description
Rabbit polyclonal to mtTFA - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Mouse mtTFA. Native recombinant mouse mtTFA protein with a C-terminal 6-His tag.
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Positive control
- Rat kidney lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab131607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 28 kDa. |
Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7019 Human
- Entrez Gene: 21780 Mouse
- Entrez Gene: 83474 Rat
- Omim: 600438 Human
- SwissProt: Q00059 Human
- SwissProt: P40630 Mouse
- SwissProt: Q91ZW1 Rat
- Unigene: 594250 Human
see all -
Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)All lanes : Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution
Lane 1 : Mouse liver whole tissue lysate
Lane 2 : Mouse hepatocytes whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Exposure time: 10 seconds
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Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution + Rat kidney lysate
Predicted band size: 28 kDa
Protocols
References
This product has been referenced in:
- Pal S et al. The osteogenic effect of liraglutide involves enhanced mitochondrial biogenesis in osteoblasts. Biochem Pharmacol 164:34-44 (2019). Read more (PubMed: 30885766) »
- Zhao Y et al. ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects. Nat Commun 10:1371 (2019). Read more (PubMed: 30914652) »
