Anti-mtTFA antibody - Mitochondrial Marker (ab131607)
Key features and details
- Rabbit polyclonal to mtTFA - Mitochondrial Marker
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-mtTFA antibody - Mitochondrial Marker
See all mtTFA primary antibodies -
Description
Rabbit polyclonal to mtTFA - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Mouse mtTFA. Native recombinant mouse mtTFA protein with a C-terminal 6-His tag.
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Positive control
- Rat kidney lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab131607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 28 kDa. |
Target
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Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
Sequence similarities
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 7019 Human
- Entrez Gene: 21780 Mouse
- Entrez Gene: 83474 Rat
- Omim: 600438 Human
- SwissProt: Q00059 Human
- SwissProt: P40630 Mouse
- SwissProt: Q91ZW1 Rat
- Unigene: 594250 Human
see all -
Alternative names
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
Images
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Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)All lanes : Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution
Lane 1 : Mouse liver whole tissue lysate
Lane 2 : Mouse hepatocytes whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Exposure time: 10 seconds
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Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab131607)Anti-mtTFA antibody - Mitochondrial Marker (ab131607) at 1/2000 dilution + Rat kidney lysate
Predicted band size: 28 kDa
References (79)
ab131607 has been referenced in 79 publications.
- Esteves P et al. Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F. Oncogene 39:617-636 (2020). PubMed: 31527668
- Kang JG et al. A Mouse Homolog of a Human TP53 Germline Mutation Reveals a Lipolytic Activity of p53. Cell Rep 30:783-792.e5 (2020). PubMed: 31968253
- Feng LR et al. Cancer-related fatigue during combined treatment of androgen deprivation therapy and radiotherapy is associated with mitochondrial dysfunction. Int J Mol Med 45:485-496 (2020). PubMed: 31894256
- Tang X et al. Evaluating the Activity of Sodium Butyrate to Prevent Osteoporosis in Rats by Promoting Osteal GSK-3ß/Nrf2 Signaling and Mitochondrial Function. J Agric Food Chem 68:6588-6603 (2020). PubMed: 32459091
- Bi J et al. Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver Injury. Oxid Med Cell Longev 2020:6946037 (2020). PubMed: 31976032
Customer reviews and Q&As
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