Product nameAnti-MTTP/MTP antibody
See all MTTP/MTP primary antibodies
DescriptionRabbit polyclonal to MTTP/MTP
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Synthetic peptide corresponding to Human MTTP/MTP aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Liver Tissue Lysate.
This product was previously labelled as MTTP
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab63467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).|
FunctionCatalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B.
Tissue specificityLiver and small intestine. Also found in ovary, testis and kidney.
Involvement in diseaseAbetalipoproteinemia
Sequence similaritiesContains 1 vitellogenin domain.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- ABL antibody
- Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
- Microsomal triglyceride transfer protein 88kD antibody
Anti-MTTP/MTP antibody (ab63467) at 1 µg/ml + Liver (Human) Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Exposure time: 20 minutes
ICC/IF image of ab63467 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63467 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5min) HeLa cell types at 1ug/ml
This product has been referenced in:
- Hu L et al. Avasimibe: A novel hepatitis C virus inhibitor that targets the assembly of infectious viral particles. Antiviral Res 148:5-14 (2017). Read more (PubMed: 29074218) »
- Yamamoto Y et al. Rapid alternative absorption of dietary long-chain fatty acids with upregulation of intestinal glycosylated CD36 in liver cirrhosis. Am J Clin Nutr 96:90-101 (2012). Human . Read more (PubMed: 22648712) »