Anti-MTTP/MTP antibody (ab75316)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-MTTP/MTP antibody
See all MTTP/MTP primary antibodies -
Description
Rabbit polyclonal to MTTP/MTP -
Host species
Rabbit -
Specificity
Reactivity with human samples appears to be lot-dependent. A user reported strong western blot reactivity with a mouse cell lysate but none with a human HepG2 lysate. -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse MTTP/MTP (C terminal).
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General notes
This product was previously labelled as MTTP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab75316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. -
Tissue specificity
Liver and small intestine. Also found in ovary, testis and kidney. -
Involvement in disease
Abetalipoproteinemia -
Sequence similarities
Contains 1 vitellogenin domain. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 17777 Mouse
- SwissProt: O08601 Mouse
- Unigene: 2941 Mouse
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Alternative names
- ABL antibody
- Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
- Microsomal triglyceride transfer protein 88kD antibody
see all
Images
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Western blot - Anti-MTTP/MTP antibody (ab75316)Anti-MTTP/MTP antibody (ab75316) at 1/500 dilution + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
References
This product has been referenced in:
- Cao XF et al. High-fat diet induces aberrant hepatic lipid secretion in blunt snout bream by activating endoplasmic reticulum stress-associated IRE1/XBP1 pathway. Biochim Biophys Acta Mol Cell Biol Lipids 1864:213-223 (2019). Read more (PubMed: 30553054) »
- Tran DD et al. Treatment with MAPKAP2 (MK2) inhibitor and DNA methylation inhibitor, 5-aza dC, synergistically triggers apoptosis in hepatocellular carcinoma (HCC) via tristetraprolin (TTP). Cell Signal 28:1872-1880 (2016). Read more (PubMed: 27619201) »
