Anti-MTTP/MTP antibody (ab75316)
Key features and details
- Rabbit polyclonal to MTTP/MTP
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-MTTP/MTP antibody
See all MTTP/MTP primary antibodies -
Description
Rabbit polyclonal to MTTP/MTP -
Host species
Rabbit -
Specificity
Reactivity with human samples appears to be lot-dependent. A user reported strong western blot reactivity with a mouse cell lysate but none with a human HepG2 lysate. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse MTTP/MTP (C terminal).
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General notes
This product was previously labelled as MTTP
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/200 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
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| Notes |
|---|
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WB
1/200 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
Target
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Function
Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B. -
Tissue specificity
Liver and small intestine. Also found in ovary, testis and kidney. -
Involvement in disease
Abetalipoproteinemia -
Sequence similarities
Contains 1 vitellogenin domain. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 17777 Mouse
- SwissProt: O08601 Mouse
- Unigene: 2941 Mouse
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Alternative names
- ABL antibody
- Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
- Microsomal triglyceride transfer protein 88kD antibody
see all
Images
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Western blot - Anti-MTTP/MTP antibody (ab75316)Anti-MTTP/MTP antibody (ab75316) at 1/500 dilution + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab75316 has been referenced in 7 publications.
- Cao XF et al. High-fat diet induces aberrant hepatic lipid secretion in blunt snout bream by activating endoplasmic reticulum stress-associated IRE1/XBP1 pathway. Biochim Biophys Acta Mol Cell Biol Lipids 1864:213-223 (2019). PubMed: 30553054
- Luo H et al. AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. Cell Metab 27:843-853.e6 (2018). PubMed: 29617643
- Tran DD et al. Treatment with MAPKAP2 (MK2) inhibitor and DNA methylation inhibitor, 5-aza dC, synergistically triggers apoptosis in hepatocellular carcinoma (HCC) via tristetraprolin (TTP). Cell Signal 28:1872-1880 (2016). PubMed: 27619201
- Hesse D et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). WB ; Mouse . PubMed: 24186947
- Lee LY et al. Activation of the Nrf2-ARE pathway in hepatocytes protects against steatosis in nutritionally induced non-alcoholic steatohepatitis in mice. Toxicol Sci 142:361-74 (2014). PubMed: 25294219
- Stancu CS et al. Probiotics determine hypolipidemic and antioxidant effects in hyperlipidemic hamsters. Mol Nutr Food Res 58:559-68 (2014). WB ; Hamster . PubMed: 24105997
- Pelz S et al. A methionine-choline-deficient diet elicits NASH in the immunodeficient mouse featuring a model for hepatic cell transplantation. Exp Cell Res : (2011). PubMed: 22108588