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Signal Transduction Metabolism Lipid metabolism
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Anti-MTTP/MTP antibody (ab75316)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Western blot - Anti-MTTP/MTP antibody (ab75316)

    Key features and details

    • Rabbit polyclonal to MTTP/MTP
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

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    Primary
    Anti-Apolipoprotein B antibody (ab31992)

    View more associated products

    Overview

    • Product name

      Anti-MTTP/MTP antibody
      See all MTTP/MTP primary antibodies
    • Description

      Rabbit polyclonal to MTTP/MTP
    • Host species

      Rabbit
    • Specificity

      Reactivity with human samples appears to be lot-dependent. A user reported strong western blot reactivity with a mouse cell lysate but none with a human HepG2 lysate.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Synthetic peptide corresponding to Mouse MTTP/MTP (C terminal).

    • General notes

       This product was previously labelled as MTTP

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: Whole serum
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipoproteins/Apolipoproteins
      • Apolipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab75316 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/200 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
    Notes
    WB
    1/200 - 1/2000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

    Target

    • Function

      Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B.
    • Tissue specificity

      Liver and small intestine. Also found in ovary, testis and kidney.
    • Involvement in disease

      Abetalipoproteinemia
    • Sequence similarities

      Contains 1 vitellogenin domain.
    • Cellular localization

      Endoplasmic reticulum.
    • Target information above from: UniProt accession P55157 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 17777 Mouse
      • SwissProt: O08601 Mouse
      • Unigene: 2941 Mouse
      • Alternative names

        • ABL antibody
        • Microsomal triglyceride transfer protein (large polypeptide 88kDa) antibody
        • Microsomal triglyceride transfer protein 88kD antibody
        • Microsomal triglyceride transfer protein antibody
        • Microsomal triglyceride transfer protein large subunit antibody
        • MTP antibody
        • MTP large subunit antibody
        • MTP_HUMAN antibody
        • MTTP antibody
        • Transfer RNA, mitochondrial, proline antibody
        • tRNA-Pro, mitochondrial antibody
        see all

      Images

      • Western blot - Anti-MTTP/MTP antibody (ab75316)
        Western blot - Anti-MTTP/MTP antibody (ab75316)
        Anti-MTTP/MTP antibody (ab75316) at 1/500 dilution + Liver (Mouse) Tissue Lysate at 10 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 99 kDa
        Observed band size: 99 kDa
        Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 1 minute

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (7)

      Publishing research using ab75316? Please let us know so that we can cite the reference in this datasheet.

      ab75316 has been referenced in 7 publications.

      • Cao XF  et al. High-fat diet induces aberrant hepatic lipid secretion in blunt snout bream by activating endoplasmic reticulum stress-associated IRE1/XBP1 pathway. Biochim Biophys Acta Mol Cell Biol Lipids 1864:213-223 (2019). PubMed: 30553054
      • Luo H  et al. AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity. Cell Metab 27:843-853.e6 (2018). PubMed: 29617643
      • Tran DD  et al. Treatment with MAPKAP2 (MK2) inhibitor and DNA methylation inhibitor, 5-aza dC, synergistically triggers apoptosis in hepatocellular carcinoma (HCC) via tristetraprolin (TTP). Cell Signal 28:1872-1880 (2016). PubMed: 27619201
      • Hesse D  et al. Hepatic trans-Golgi action coordinated by the GTPase ARFRP1 is crucial for lipoprotein lipidation and assembly. J Lipid Res 55:41-52 (2014). WB ; Mouse . PubMed: 24186947
      • Lee LY  et al. Activation of the Nrf2-ARE pathway in hepatocytes protects against steatosis in nutritionally induced non-alcoholic steatohepatitis in mice. Toxicol Sci 142:361-74 (2014). PubMed: 25294219
      • Stancu CS  et al. Probiotics determine hypolipidemic and antioxidant effects in hyperlipidemic hamsters. Mol Nutr Food Res 58:559-68 (2014). WB ; Hamster . PubMed: 24105997
      • Pelz S  et al. A methionine-choline-deficient diet elicits NASH in the immunodeficient mouse featuring a model for hepatic cell transplantation. Exp Cell Res : (2011). PubMed: 22108588

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-MTTP/MTP antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Hamster Tissue lysate - whole (Hamster (Mesocricetus auratus) small intestine)
      Gel Running Conditions
      Reduced Denaturing (10% SDS-PAGE)
      Loading amount
      75 µg
      Treatment
      Western diet (N normal, HL hyperlipemic), 16 weeks
      Specification
      Hamster (Mesocricetus auratus) small intestine
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Dr. Anca Sima

      Verified customer

      Submitted May 21 2015

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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