Product nameAnti-Mu Opioid Receptor antibody
See all Mu Opioid Receptor primary antibodies
DescriptionRabbit polyclonal to Mu Opioid Receptor
Tested applicationsSuitable for: ICC/IF, WB, IHC-Fr, ICC, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Guinea pig, Human
Predicted to work with: Cow, Pig, Non human primates, Macaque monkey
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab10275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/2000.|
|WB||1/500 - 1/1000. Detects a band of approximately 49-65 kDa (predicted molecular weight: 45 kDa).Can be blocked with Rat Mu Opioid Receptor peptide (ab46988).
Approximately 49 -65 kDa depending on the tissue lysate. Can be blocked with ab46988.
|IHC-Fr||1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.|
|ICC||1/100 - 1/2000.|
|IHC-FoFr||1/100 - 1/2000. We recommend incubating for 18-24 hours at 4C.|
FunctionInhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of rat brain tissue, staining Mu Opioid Receptor with ab10275.
Tissue was blocked with 5% normal donkey serum and 0.4% Triton X-100 for 1 hour at room temperature. Sections were incubated with primary antibody (1/2000) for 24 hours at 4°C. An AlexaFluor®594-conjugated donkey anti-rabbit IgG (1/250) was used as the secondary antibody.
Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.
This product has been referenced in:
- Hou X et al. Suppression of HDAC2 in Spinal Cord Alleviates Mechanical Hyperalgesia and Restores KCC2 Expression in a Rat Model of Bone Cancer Pain. Neuroscience 377:138-149 (2018). WB ; Rat . Read more (PubMed: 29482000) »
- Wu ZY et al. Endomorphin-2 Decreases Excitatory Synaptic Transmission in the Spinal Ventral Horn of the Rat. Front Neural Circuits 11:55 (2017). Read more (PubMed: 28848403) »