Overview

  • Product name
    Anti-Mu Opioid Receptor antibody
    See all Mu Opioid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Mu Opioid Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) (Internal sequence,

    ARIRQNTREHPSTAC

    ).

  • Positive control
    • brain, spinal cord (lysate or tissue)

Properties

Applications

Our Abpromise guarantee covers the use of ab17934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 45 kDa.

Target

Images

  • Western Blot for ab17934

    Extracts prepared from rat brain lysates were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with ab17934 µ-opioid receptor pan antibody (1/1000) for 2h at RT in 1% BSA-TBST buffer. After washing, the membrane was incubated with goat anti-rabbit IgG HRP conjugate. Bands were detected using the Pierce SuperSignal™ method.

    Western Blot for ab17934

    Extracts prepared from rat brain lysates were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with ab17934 µ-opioid receptor pan antibody (1/1000) for 2h at RT in 1% BSA-TBST buffer. After washing, the membrane was incubated with goat anti-rabbit IgG HRP conjugate. Bands were detected using the Pierce SuperS

References

This product has been referenced in:
  • Hubbs AF  et al. Accumulation of Ubiquitin and Sequestosome-1 Implicate Protein Damage in Diacetyl-Induced Cytotoxicity. Am J Pathol N/A:N/A (2016). ICC/IF ; Mouse . Read more (PubMed: 27643531) »
  • Leoncikas V  et al. Generation of 2,000 breast cancer metabolic landscapes reveals a poor prognosis group with active serotonin production. Sci Rep 6:19771 (2016). Read more (PubMed: 26813959) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Gracias por contactarnos.
Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que ninguno de ellos ha demostrado reconocer el epítopo extracelular de la proteína.
Todos ellos se han generado a partir del extremo C terminal de la proteína, por lo que, a pesar de no haberse mapeado el epítopo que reconocen, probablemente detecten el dominio citoplásmico de la misma.
La solución que te propongo es testar uno de los anticuerpos que hay en el catálogo hecho a partir del extremo N terminal de la proteína, y testarlo en rata. Tenemos en abcam un sistema de evaluación de anticuerpos por el cual al testar uno de ellos en una aplicación o especie que no se ha testado, puedes beneficiarte de un descuento en tu próxima compra por valor del anticuerpo, si lo usas en esa especie o aplicación nueva y nos mandas los resultados a la web en forma de Abreview.
Para saber más sobre este sistema: www.abcam.com/abtrial
De las posibles alternativas, ab140911, ab137460 o ab137277 indícame si quieres cuál os interesaría más, para averiguar su homología con la proteína en rata y determinar la posibilidad de reaccionar en esta especie, en caso de que queráis participar en el sistema Abtrial.
Espero haberte ayudado. No dudes en contactarnos de nuevo para cualquier otra consulta.

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Answer

Thank you for contacting us.
We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and this antibody (as indicated on the datasheet) has not been tested for IHC. We are therefore unable to provide any specific recommendation or apply our guarantee for this particular product in this specific application.
We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests.
I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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Answer

Thank you for this information. Although your samples are showing receptor expressio with another product, Abcam antibodies will have been tested under reduced and denatured conditions only and we recommend using reducing and denaturing conditions unless otherwise stated on the datasheet. You may need to change your conditions in order to improve your result with this product. Please let me know if you have any further questions.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
80 µg
Specification
HEK293 cells
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 01 2010

Answer

Thank you for stopping by the Abcam booth at SFN, my colleague Sarah passed your enquiry onto me. I'm sorry that you didn't receive our response back in June. Presently, ab17934 is currently in stock. The batches that we have are batches 129573, 129620, and 132645. What was the batch number that you experienced problems with? Please let me know if you have any additional questions, and enjoy the rest of the conference!

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Answer

Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. As we understand from your e-mail, the customer used mouse Mu Opioid Receptor expressing HEK cells. We would like to emphasize that this antibody was rtaised against synthetic peptide of human Mu Opioid Receptor. Therefore the antibody recognizes human protein, but unfortunately other species i.e. mouse has not been tested for cross-reaction. Although, there is a chance that it may work in this species due to 94% sequence homology. 1. We would suggest running a good positive control along with the samples, lysate from human brain or spinal cord would be a good candidate. 2. We do not have information about the Bock Ace, so would rather suggest using 5% BSA-TBST for blocking. 3. Please make sure that the customer use RIPA buffer for sample preparation: RIPA Base Ingredients Tris-HCl: 50 mM, pH 7.4 NP-40: 1% Na-deoxycholate: 0.25% NaCl: 150 mM EDTA: 1 mM PMSF: 1 mM Aprotinin, leupeptin, pepstatin: 1 microgram/ml each RIPA Protease Inhibitors Phenylmethylsulfonyl fluoride (PMSF) (200 mM stock solution in isopropanol; store at room temperature) EDTA (calcium chelator; 100 mM stock solution in H2O, pH 7.4) Leupeptin (store frozen in aliquots, 1 mg/ml in H2O) Aprotinin (store frozen in aliquots, 1 mg/ml in H2O) Pepstatin (store frozen in aliquots, 1 mg/ml in methanol) We hope this information will be useful. Should your customer still have problem, then please do not hesitate to contact us again.

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Question
Answer

Thank you for your enquiry. The peptide sequence is :Ac-Ala-Arg-Ile-Arg-Gln-Asn-Thr-Arg-Glu-His-Pro-Ser-Thr-Ala-Cys-NH2 and this information has been added to the datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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