Recombinant Anti-Mu Opioid Receptor antibody [EPR26293-61] (ab302512)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)](https://www.abcam.com/ps/products/302/ab302512/Images/AB302512-5-anti-mu-opioid-receptor-antibody-epr2629361-immunohistochemistry-transfected-hek293t-cells1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26293-61] to Mu Opioid Receptor
- Suitable for: IHC-P
- Reacts with: Rat
Overview
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Product name
Anti-Mu Opioid Receptor antibody [EPR26293-61]
See all Mu Opioid Receptor primary antibodies -
Description
Rabbit monoclonal [EPR26293-61] to Mu Opioid Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC/IF,IHC-Fr,IP or WB -
Species reactivity
Reacts with: Rat
Does not react with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Rat spinal cord and cerebrum FFPE tissue sections; HEK-293T cells transfected with a rat OPRM1 expression vector containing a myc-His tag cell pellet.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26293-61 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 25601 Rat
- SwissProt: P33535 Rat
- Unigene: 10118 Rat
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Alternative names
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)
Immunohistochemical analysis of paraffin-embedded HEK-293T cells labeling Mu Opioid Receptor. The section was incubated with ab302512 for 30 mins at room temperature at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Cytoplasmic staining on A: HEK-293T cells transfected with a rat OPRM1 expression vector containing a myc-His tag cell pellet, and no staining on B: 293T transfected with an empty vector cell pellet. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)](https://www.abcam.com/ps/products/302/ab302512/Images/AB302512-4-anti-mu-opioid-receptor-antibody-epr2629361-immunohistochemistry-rat-liver1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Mu Opioid Receptor with ab302512 at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Negative control: no staining on rat liver. The section was incubated with ab302512 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)](https://www.abcam.com/ps/products/302/ab302512/Images/AB302512-3-anti-mu-opioid-receptor-antibody-epr2629361-immunohistochemistry-rat-cerebrum1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Mu Opioid Receptor with ab302512 at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on rat cerebrum. The section was incubated with ab302512 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)](https://www.abcam.com/ps/products/302/ab302512/Images/AB302512-2-anti-mu-opioid-receptor-antibody-epr2629361-immunohistochemistry-rat-spinal-cord1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling Mu Opioid Receptor with ab302512 at 1/500 dilution (0.924 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on rat spinal cord. The section was incubated with ab302512 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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![Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)](https://www.abcam.com/ps/products/302/ab302512/Images/AB302512-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Mu Opioid Receptor antibody [EPR26293-61] (AB302512)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab302512 has not yet been referenced specifically in any publications.