Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-Mu Opioid Receptor antibody [UMB3] - C-terminal
See all Mu Opioid Receptor primary antibodies
DescriptionRabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal
The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.
This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Mu Opioid Receptor aa 350-450 (C terminal). The exact sequence is proprietary.
Database link: P35372
- WB: Mu Opioid Receptor transfected HEK-293 lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab134054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).|
FunctionInhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution
Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
Lane 2 : HEK-293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with ab134054.
Arrows show nonspecific bands, presumably of rabbit IgG.
For more protocol information please see PMID: 20851148.
Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with another anti Mu Opioid Receptor antibody. Incubations were performed in the absence (−) or presence (+) of the peptide antigen.
For more protocol information please see PMID: 20851148.
ab134054 has been referenced in 25 publications.
- DiCello JJ et al. Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon. Cell Mol Gastroenterol Hepatol 9:465-483 (2020). PubMed: 31759144
- Zhang XY et al. Mu-Opioid Receptors Expressed in Glutamatergic Neurons are Essential for Morphine Withdrawal. Neurosci Bull N/A:N/A (2020). PubMed: 32451910
- Arttamangkul S et al. Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry. Elife 8:N/A (2019). PubMed: 31589142
- Popova D et al. Synaptic Regulation by OPRM1 Variants in Reward Neurocircuitry. J Neurosci 39:5685-5696 (2019). PubMed: 31109961
- Ehrlich AT et al. Biased Signaling of the Mu Opioid Receptor Revealed in Native Neurons. iScience 14:47-57 (2019). PubMed: 30925410