Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)

Reviews (1)Q&A (4)References (25)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal
  • Suitable for: WB, IP, ICC/IF, IHC-P, IHC-FoFr
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal
    See all Mu Opioid Receptor primary antibodies

  • Description

    Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal

  • Host species

    Rabbit

  • Specificity

    The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.

    This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.

  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-P, IHC-FoFrmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Mu Opioid Receptor aa 350-450 (C terminal). The exact sequence is proprietary.
    Database link: P35372

  • Positive control

    • WB: Mu Opioid Receptor transfected HEK-293 lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab134054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).
IP Use at an assay dependent concentration. PubMed: 20851148
ICC/IF Use at an assay dependent concentration. PubMed: 20851148
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 20851148
IHC-FoFr Use at an assay dependent concentration. PubMed: 28092666
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    Images

    • Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
      Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
      All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution

      Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
      Lane 2 : HEK-293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
      Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

      Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with ab134054.

      Arrows show nonspecific bands, presumably of rabbit IgG.

      For more protocol information please see PMID: 20851148.

    • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
      Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

      Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with another anti Mu Opioid Receptor antibody. Incubations were performed in the absence (−) or presence (+) of the peptide antigen.

      For more protocol information please see PMID: 20851148.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 2.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

      Immunohistochemical localization of MOR in rat hippocampus (E), striatum (F) and nucleus accumbens (G), mouse dorsal root ganglia (H) and gallbladder (I), and human enteric ganglia (J). Sections were dewaxed, microwaved in citric acid and incubated with ab134054.

      For more protocol information please see PMID: 20851148.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

      Immunohistochemical staining of brain sections from MOR-deficient mice (MOR−/−) and their wild-type littermates (MOR+/+). Brain sections were dewaxed, microwaved in citric acid and incubated with ab134054. Sections were sequentially treated with biotinylated anti-rabbit IgG, peroxidase-conjugated avidin and developed in AEC.

      For more protocol information please see PMID: 20851148.

    • Immunocytochemistry - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
      Immunocytochemistry - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

      Immunocytochemistry of rat MOR-transfected HEK-293 cells. Cells were exposed for 30 min to etonitazene (D) or left untreated (C), subsequently fixed and immunofluorescently stained with ab134054. Note that in untreated cells prominent immunofluorescence was localized at the level of the plasma membrane and that etonitazene exposure induced a rapid translocation of MOR receptors from the plasma membrane into the cytosol.

      For more protocol information please see PMID: 20851148.

    References

    Publishing research using ab134054? Please let us know so that we can cite the reference in this datasheet.

    ab134054 has been referenced in 25 publications.

    • DiCello JJ  et al. Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon. Cell Mol Gastroenterol Hepatol 9:465-483 (2020). PubMed: 31759144
    • Zhang XY  et al. Mu-Opioid Receptors Expressed in Glutamatergic Neurons are Essential for Morphine Withdrawal. Neurosci Bull N/A:N/A (2020). PubMed: 32451910
    • Arttamangkul S  et al. Visualizing endogenous opioid receptors in living neurons using ligand-directed chemistry. Elife 8:N/A (2019). PubMed: 31589142
    • Popova D  et al. Synaptic Regulation by OPRM1 Variants in Reward Neurocircuitry. J Neurosci 39:5685-5696 (2019). PubMed: 31109961
    • Ehrlich AT  et al. Biased Signaling of the Mu Opioid Receptor Revealed in Native Neurons. iScience 14:47-57 (2019). PubMed: 30925410
    View all Publications for this product

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (40 µm brain section from cryostat)
    Permeabilization
    Yes - 0.4% triton
    Specification
    40 µm brain section from cryostat
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
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    Abcam user community

    Verified customer

    Submitted Feb 06 2020

    Question

    A quien corresponda,
    Estoy interesada en un anticuerpo anti-receptor mu-opioide de rata que reconozca un epitope extracelular. He encontrado dos anticuerpos en el sitio web que reconocen el extremo N-terminal, pero del receptor humano, y hay otros dos, que reconocen rata, pero cuyos inmunógenos son péptidos sintéticos de os cuales no se aclara exactamente la posición.
    La información que se detalla es la siguiente:
    Anti-Mu Opioid Receptor antibody (ab17934)
    Immunogen
    Synthetic peptide (Human) (Internal sequence, ARIRQNTREHPSTAC).
    ARIRQNTREHPSTAC
    Anti-Mu Opioid Receptor antibody (ab64746)
    Immunogen
    Synthetic peptide: NHQLENLEAETAPLP
    NHQLENLEAETAPLP
    Le estaría sumamente agradecida si pudieran por favor indicarme si alguno de estos dos anticuerpos sería válido para mi objetivo.
    Muchas gracias desde ya y un cordial saludo,

    Read More
    Answer

    Gracias por contactarnos.
    Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que ninguno de ellos ha demostrado reconocer el epítopo extracelular de la proteína.
    Todos ellos se han generado a partir del extremo C terminal de la proteína, por lo que, a pesar de no haberse mapeado el epítopo que reconocen, probablemente detecten el dominio citoplásmico de la misma.
    La solución que te propongo es testar uno de los anticuerpos que hay en el catálogo hecho a partir del extremo N terminal de la proteína, y testarlo en rata. Tenemos en abcam un sistema de evaluación de anticuerpos por el cual al testar uno de ellos en una aplicación o especie que no se ha testado, puedes beneficiarte de un descuento en tu próxima compra por valor del anticuerpo, si lo usas en esa especie o aplicación nueva y nos mandas los resultados a la web en forma de Abreview.
    Para saber más sobre este sistema: www.abcam.com/abtrial
    De las posibles alternativas, ab140911, ab137460 o ab137277 indícame si quieres cuál os interesaría más, para averiguar su homología con la proteína en rata y determinar la posibilidad de reaccionar en esta especie, en caso de que queráis participar en el sistema Abtrial.
    Espero haberte ayudado. No dudes en contactarnos de nuevo para cualquier otra consulta.

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    Question

    ab83774, ab103463: tested in dog, send discount code
    ab46545: try in dog, send discount code

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    Answer

    Thank you for your phone call. I have created the following discount codes for you:


    DISCOUNT CODE: xxx for ab83774
    DISCOUNT CODE: xxx for ab103463
    DISCOUNT CODE: xxx for ab46545

    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab83774, ab103463 and ab46545 in dog. Each code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for dogand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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    Question

    One more question if you don't mind!

    Could you please let me know the highest concentration level of AB134054 you currently have available in stock, as our customer is interested in obtaining the AB134054 with the highest level of concentration.

    I look forward to hearing back from you!

    Enjoy your weekend

    Kind regards,

    Read More
    Answer

    Thanks for your message.

    I am sorry to confirm, as stated in my last emails that we do not have this product in stock at the moment, so regrettably I am not able to let you know what the concentration for the current stock is.

    I am sorry I am not able to provide more information on this occasion. If you have any further questions, please do not hesitate to contact us.

    Have a great weekend.

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    Question

    As per our customer request could you please advise the current lot’s concentration of the above antibody that you have in stock.

    Hoping to hear back from you soon.

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    Answer



    Unfortunately, I cannot answer this question: the new lot is in production right now! Or does your customer have a specificlot he is interested in?

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