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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)

Reviews (2)Q&A (4)References (30)
Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
  • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
  • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
  • Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal
    See all Mu Opioid Receptor primary antibodies

  • Description

    Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal

  • Host species

    Rabbit

  • Specificity

    The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.

    This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Mu Opioid Receptor aa 350-450 (C terminal). The exact sequence is proprietary.
    Database link: P35372

  • Positive control

    • WB: Mu Opioid Receptor transfected HEK-293 lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).
Application notes
Is unsuitable for Flow Cyt.

Target

Images

  • Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution

    Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
    Lane 2 : HEK-293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

    Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with ab134054.

    Arrows show nonspecific bands, presumably of rabbit IgG.

    For more protocol information please see PMID: 20851148.

  • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

    Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with another anti Mu Opioid Receptor antibody. Incubations were performed in the absence (−) or presence (+) of the peptide antigen.

    For more protocol information please see PMID: 20851148.

  • Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)

References (30)

Publishing research using ab134054? Please let us know so that we can cite the reference in this datasheet.

ab134054 has been referenced in 30 publications.

  • Zhang HW  et al. Morphine Suppresses Liver Cancer Cell Tumor Properties In Vitro and In Vivo. Front Oncol 11:666446 (2021). PubMed: 33968773
  • Morigaki R  et al. Spatiotemporal Up-Regulation of Mu Opioid Receptor 1 in Striatum of Mouse Model of Huntington's Disease Differentially Affecting Caudal and Striosomal Regions. Front Neuroanat 14:608060 (2020). PubMed: 33362481
  • DiCello JJ  et al. Mu and Delta Opioid Receptors Are Coexpressed and Functionally Interact in the Enteric Nervous System of the Mouse Colon. Cell Mol Gastroenterol Hepatol 9:465-483 (2020). PubMed: 31759144
  • Zhang XY  et al. Mu-Opioid Receptors Expressed in Glutamatergic Neurons are Essential for Morphine Withdrawal. Neurosci Bull N/A:N/A (2020). PubMed: 32451910
  • Zhang XY  et al. Different neuronal populations mediate inflammatory pain analgesia by exogenous and endogenous opioids. Elife 9:N/A (2020). PubMed: 32519950
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal

 Good
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain, striatum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6
Permeabilization
Yes - 0.2 Triton X-100
Specification
Brain, striatum
Blocking step
normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 10 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (40 µm brain section from cryostat)
Permeabilization
Yes - 0.4% triton
Specification
40 µm brain section from cryostat
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 06 2020

Question

A quien corresponda,
Estoy interesada en un anticuerpo anti-receptor mu-opioide de rata que reconozca un epitope extracelular. He encontrado dos anticuerpos en el sitio web que reconocen el extremo N-terminal, pero del receptor humano, y hay otros dos, que reconocen rata, pero cuyos inmunógenos son péptidos sintéticos de os cuales no se aclara exactamente la posición.
La información que se detalla es la siguiente:
Anti-Mu Opioid Receptor antibody (ab17934)
Immunogen
Synthetic peptide (Human) (Internal sequence, ARIRQNTREHPSTAC).
ARIRQNTREHPSTAC
Anti-Mu Opioid Receptor antibody (ab64746)
Immunogen
Synthetic peptide: NHQLENLEAETAPLP
NHQLENLEAETAPLP
Le estaría sumamente agradecida si pudieran por favor indicarme si alguno de estos dos anticuerpos sería válido para mi objetivo.
Muchas gracias desde ya y un cordial saludo,

Read More
Answer

Gracias por contactarnos.
Después de consultar este caso con mis compañeros del laboratorio, acerca de los siguientes anticuerpos contra Mu Opioid Receptor validados en tejidos de rata: ab10275, ab17934, ab64746, ab128013 y ab134054, me temo que ninguno de ellos ha demostrado reconocer el epítopo extracelular de la proteína.
Todos ellos se han generado a partir del extremo C terminal de la proteína, por lo que, a pesar de no haberse mapeado el epítopo que reconocen, probablemente detecten el dominio citoplásmico de la misma.
La solución que te propongo es testar uno de los anticuerpos que hay en el catálogo hecho a partir del extremo N terminal de la proteína, y testarlo en rata. Tenemos en abcam un sistema de evaluación de anticuerpos por el cual al testar uno de ellos en una aplicación o especie que no se ha testado, puedes beneficiarte de un descuento en tu próxima compra por valor del anticuerpo, si lo usas en esa especie o aplicación nueva y nos mandas los resultados a la web en forma de Abreview.
Para saber más sobre este sistema: www.abcam.com/abtrial
De las posibles alternativas, ab140911, ab137460 o ab137277 indícame si quieres cuál os interesaría más, para averiguar su homología con la proteína en rata y determinar la posibilidad de reaccionar en esta especie, en caso de que queráis participar en el sistema Abtrial.
Espero haberte ayudado. No dudes en contactarnos de nuevo para cualquier otra consulta.

Read More

Question

ab83774, ab103463: tested in dog, send discount code
ab46545: try in dog, send discount code

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Answer

Thank you for your phone call. I have created the following discount codes for you:


DISCOUNT CODE: xxx for ab83774
DISCOUNT CODE: xxx for ab103463
DISCOUNT CODE: xxx for ab46545

Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab83774, ab103463 and ab46545 in dog. Each code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for dogand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Question

One more question if you don't mind!

Could you please let me know the highest concentration level of AB134054 you currently have available in stock, as our customer is interested in obtaining the AB134054 with the highest level of concentration.

I look forward to hearing back from you!

Enjoy your weekend

Kind regards,

Read More
Answer

Thanks for your message.

I am sorry to confirm, as stated in my last emails that we do not have this product in stock at the moment, so regrettably I am not able to let you know what the concentration for the current stock is.

I am sorry I am not able to provide more information on this occasion. If you have any further questions, please do not hesitate to contact us.

Have a great weekend.

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Question

As per our customer request could you please advise the current lot’s concentration of the above antibody that you have in stock.

Hoping to hear back from you soon.

Read More
Answer



Unfortunately, I cannot answer this question: the new lot is in production right now! Or does your customer have a specificlot he is interested in?

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