Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB3] to Mu Opioid Receptor - Low endotoxin, Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Mu Opioid Receptor antibody [UMB3] - Low endotoxin, Azide free
See all Mu Opioid Receptor primary antibodies
DescriptionRabbit monoclonal [UMB3] to Mu Opioid Receptor - Low endotoxin, Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to C terminal residues of Human Mu Opioid Receptor.
- Mu Opioid Receptor transfected HEK293 lysate.
ab227067 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab227067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 75-78 kDa (predicted molecular weight: 45 kDa).|
FunctionInhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- hMOP antibody
- LMOR antibody
- M-OR-1 antibody
All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution
Lane 1 : Mu Opioid Receptor transfected HEK293 lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227067 has been referenced in 8 publications.
- Crittenden JR et al. Striosome-dendron bouquets highlight a unique striatonigral circuit targeting dopamine-containing neurons. Proc Natl Acad Sci U S A 113:11318-11323 (2016). IHC-Fr ; Mouse . PubMed: 27647894
- Ozawa A et al. Knock-In Mice with NOP-eGFP Receptors Identify Receptor Cellular and Regional Localization. J Neurosci 35:11682-93 (2015). PubMed: 26290245
- Hassan B et al. Alteration of the mu opioid receptor: Ca2+ channel signaling pathway in a subset of rat sensory neurons following chronic femoral artery occlusion. J Neurophysiol 112:3104-15 (2014). PubMed: 25231620
- Just S et al. Differentiation of opioid drug effects by hierarchical multi-site phosphorylation. Mol Pharmacol 83:633-9 (2013). PubMed: 23239825
- Gallagher SK et al. Dopaminergic amacrine cells express opioid receptors in the mouse retina. Vis Neurosci 29:203-9 (2012). WB ; Mouse . PubMed: 22643230
- Lupp A et al. UMB-3, a novel rabbit monoclonal antibody, for assessing µ-opioid receptor expression in mouse, rat and human formalin-fixed and paraffin-embedded tissues. Regul Pept 167:9-13 (2011). PubMed: 20851148
- Doll C et al. Agonist-selective patterns of µ-opioid receptor phosphorylation revealed by phosphosite-specific antibodies. Br J Pharmacol 164:298-307 (2011). PubMed: 21449911
- Grecksch G et al. Analgesic tolerance to high-efficacy agonists but not to morphine is diminished in phosphorylation-deficient S375A µ-opioid receptor knock-in mice. J Neurosci 31:13890-6 (2011). IP, IHC-P ; Mouse . PubMed: 21957251