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    muc1-antibody-ab15481.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-MUC1 antibody (ab15481)

  • Datasheet
  • SDS
Reviews (8)Q&A (6)References (27)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab15481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab15481)
  • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody (ab15481)

Key features and details

  • Rabbit polyclonal to MUC1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MUC1 antibody
    See all MUC1 primary antibodies
  • Description

    Rabbit polyclonal to MUC1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human MUC1 aa 1200 to the C-terminus. The exact sequence is proprietary.
    Database link: P15941

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human MUC1 protein (ab80082)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
All applications
Mouse
Application Abreviews Notes
ICC/IF (2)
Use a concentration of 1 µg/ml.
IHC-P (5)
1/100. Antigen retrieval is not essential but may optimise staining.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/100. Antigen retrieval is not essential but may optimise staining.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
      The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
    • Tissue specificity

      Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
    • Involvement in disease

      MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
      Medullary cystic kidney disease 1
    • Sequence similarities

      Contains 1 SEA domain.
    • Developmental stage

      During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
    • Post-translational
      modifications

      Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
      Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
      Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
      Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
      The N-terminal sequence has been shown to begin at position 24 or 28.
    • Cellular localization

      Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
    • Target information above from: UniProt accession P15941 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4582 Human
      • Entrez Gene: 17829 Mouse
      • Omim: 158340 Human
      • SwissProt: P15941 Human
      • SwissProt: Q02496 Mouse
      • Unigene: 89603 Human
      • Unigene: 16193 Mouse
      • Alternative names

        • ADMCKD antibody
        • ADMCKD1 antibody
        • Breast carcinoma associated antigen DF3 antibody
        • Breast carcinoma-associated antigen DF3 antibody
        • CA 15-3 antibody
        • CA15 3 antibody
        • CA15 3 antigen antibody
        • CA15-3 antibody
        • CA15.3 antibody
        • Cancer antigen 15-3 antibody
        • Carcinoma associated mucin antibody
        • Carcinoma-associated mucin antibody
        • CD 227 antibody
        • CD227 antibody
        • DF3 antigen antibody
        • EMA antibody
        • Episialin antibody
        • Epithelial Membrane Antigen antibody
        • H23 antigen antibody
        • H23AG antibody
        • KL 6 antibody
        • KL-6 antibody
        • KL6 antibody
        • Krebs von den Lungen-6 antibody
        • MAM 6 antibody
        • MAM6 antibody
        • MCD antibody
        • MCKD antibody
        • MCKD1 antibody
        • Medullary cystic kidney disease 1 (autosomal dominant) antibody
        • Medullary cystic kidney disease, autosomal dominant antibody
        • MUC 1 antibody
        • MUC-1 antibody
        • MUC-1/SEC antibody
        • MUC-1/X antibody
        • MUC1 antibody
        • MUC1-alpha antibody
        • MUC1-beta antibody
        • MUC1-CT antibody
        • MUC1-NT antibody
        • MUC1/ZD antibody
        • MUC1_HUMAN antibody
        • Mucin 1 antibody
        • Mucin 1 cell surface associated antibody
        • Mucin 1 transmembrane antibody
        • Mucin 1, cell surface associated antibody
        • Mucin-1 subunit beta antibody
        • Peanut reactive urinary mucin antibody
        • Peanut-reactive urinary mucin antibody
        • PEM antibody
        • PEMT antibody
        • Polymorphic epithelial mucin antibody
        • PUM antibody
        • Tumor associated epithelial membrane antigen antibody
        • Tumor associated epithelial mucin antibody
        • Tumor associated mucin antibody
        • Tumor-associated epithelial membrane antigen antibody
        • Tumor-associated mucin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab15481)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab15481)

        Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MUC1 with ab15481.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab15481)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab15481)This image is courtesy of an anonymous Abreview

        ab15481 at 1/200 staining mouse mammary gland tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with BSA. A heat mediated antigen retrieval step was performed. The antibody was incubated with the tissue for 16 hours and then an Alexa-Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary. MUC1 staining (luminal cells) is shown in green. The nuclei were counterstained with DAPI and staining is shown in blue.

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody (ab15481)
        Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody (ab15481)

        ICC/IF image of ab15481 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15481, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (27)

      Publishing research using ab15481? Please let us know so that we can cite the reference in this datasheet.

      ab15481 has been referenced in 27 publications.

      • Yu XX  et al. Defining multistep cell fate decision pathways during pancreatic development at single-cell resolution. EMBO J 38:N/A (2019). PubMed: 30737258
      • Huang L  et al. Traditional Chinese Medicine Dingkun Pill facilitates uterine receptivity for implantation in mice. Biol Reprod N/A:N/A (2019). PubMed: 31347662
      • Lee H  et al. A New Ophthalmic Pharmaceutical Formulation, Topical Sulglycotide, Enhances the Ocular Mucin Secretion in Desiccation Stress-Mediated Dry Eye Disease. Invest Ophthalmol Vis Sci 60:1076-1087 (2019). PubMed: 30901389
      • Liu B  et al. LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway. J Exp Clin Cancer Res 37:316 (2018). PubMed: 30547804
      • Shibata W  et al. Overexpression of HER2 in the pancreas promotes development of intraductal papillary mucinous neoplasms in mice. Sci Rep 8:6150 (2018). PubMed: 29670173
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 14 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-MUC1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (sebocytes)
      Permeabilization
      Yes - PBS 0,5%tween
      Specification
      sebocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 24 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Kidney)
      Antigen retrieval step
      None
      Permeabilization
      Yes - Triton 0,3% - 5 minutes
      Specification
      Kidney
      Blocking step
      Normal Goat Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 22 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Virgin tissue)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate 95C
      Permeabilization
      Yes - PBS with 3% Triton
      Specification
      Virgin tissue
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 03 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-MUC1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Breast -MCF7)
      Permeabilization
      Yes - 0.25% Triton, 15 min
      Specification
      Breast -MCF7
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 22 2015

      Question

      No siganl in IHC-P on mouse tissue.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 18 2012

      Answer

      Thank you for contacting Abcam.
      I am sorry about the issues you have been having with ab15481 in IHC-P. As we discussed I am sending you a new vial of this antibody from a different lot as a free of charge replacement. Your new order number is *******.
      If there is anything else I can help you with, please let me know.

      Read More

      Abcam Scientific Support

      Answered on Jun 18 2012

      Question

      Dear Abcam, i would like to perform an ELISA sandwich assay using this antibodies: 1) Anti-MUC1 antibody C595 NCRC48, Mouse monoclonal, (ab28081) 2) Anti-MUC1 antibody, Rabbit polyclonal, (ab15481) i've to question: 1) Can these antibodies use together? 2) Can you suggest me a compatible MUC1 protein? with my best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 21 2011

      Answer

      Sorry for the delay in contacting you with regards to this query. We have a number of pairs of mouse monoclonals which can be used to detect the under or unglycosylated form of MUC1 (for example ab10117 which can be paired with HRP conjugated ab24471). However if you are interested in the glycosylated form of MUC1, unfortunately we have not tested any antibody pairs in order to detect this form and therefore cannot guarantee that it will work. However, form the pair which you presented, ab28081 has been tested in ELISA with human protein and should therefore be suitable for this application. However, I cannot be sure that the rabbit polyclonal ab15481 will work as it has not been tested in ELISA before and has only been used in applications where the immunogen may be in the denatured form. Two possible alternative would be ab79226 which recognises the Y1229 phosphorylated form (cytoplasmic) or ab14690 which again recognises the underglycosylated form (extracellular) but have both been tested using ELISA. Alternatively, ab80952 is an Armenian Hamster monoclonal reactive against the cytoplasmic tail of MUC1, or ab37435 which is a rabbit polyclonal, again directed against the cytoplasmic domain. Alternatively ab45167 is a rabbit monoclonal which recognises the extracellular domain but only of isoform 7 or MUC1. These have been used in flow cytometry and immunoprecipitation and are therefore more likely to be able to detect the native form of the protein. If you would like to test ab15481 as you suggested (or any of the other antibodies suggested which have not been used in ELISA yet), as it has not been used in ELISA before I can offer a testing discount. This would involve you purchasing the antibody, using it and letting us know the results via an Abreview (positive or negative), you would then be eligible for a free primary antibody of your choice from our catalog. More information on this offer can be found here: www.abcam.com/collaborationdiscount If you need a secondary antibody for this application we have a very easy to use search engine to allow you to find exactly what you are looking for: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 If you would like any further information, help in choosing a suitable secondary antibody or you are interested in applying for the testing discount please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Oct 21 2011

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Mammary gland)
      Specification
      Mammary gland
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate pH6
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 17 2010

      Immunohistochemistry (Frozen sections) abreview for Anti-MUC1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Pancreas)
      Specification
      Pancreas
      Fixative
      Formaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 25 2010

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Mouse Nasal Epithelium)
      Specification
      Mouse Nasal Epithelium
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 10%
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 02 2009

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (mammary gland)
      Specification
      mammary gland
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 09 2007

      1-10 of 14 Abreviews or Q&A

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