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    muc1-antibody-bc-2-ab89492.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-MUC1 antibody [BC-2] (ab89492)

  • Datasheet
Submit a review Q&A (1)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [BC-2] (ab89492)

    Key features and details

    • Mouse monoclonal [BC-2] to MUC1
    • Suitable for: IHC-Fr, IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-MUC1 antibody [BC-2]
      See all MUC1 primary antibodies
    • Description

      Mouse monoclonal [BC-2] to MUC1
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Fr, IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human MUC1. Human milk fat globulin membrane preparation

    • Epitope

      Reacts with a 5 amino acid epitope of the MUC1 core protein which is less affective for glycosilation than the epitopes detected by other MUC1 antibodies.
    • Positive control

      • Breast carcinoma tissue IHC-P: Human pancreas adenocarcinoma tissue sections.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.5
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      BC-2
    • Isotype

      IgG1
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • MUC
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MUC
      • Cancer
      • Tumor immunology
      • Tumor-associated antigens

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human MUC1 protein (ab80082)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab89492 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    IHC-P
    Notes
    Application notes
    IHC-P: 1/20.
    IHC-Fr: 1/20.
    Note: suggested dilution based on the indirect method. With a normal ABC method the dilution can be 5-10x higher.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Function

      The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
      The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
    • Tissue specificity

      Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
    • Involvement in disease

      MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
      Medullary cystic kidney disease 1
    • Sequence similarities

      Contains 1 SEA domain.
    • Developmental stage

      During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
    • Post-translational
      modifications

      Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
      Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
      Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
      Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
      The N-terminal sequence has been shown to begin at position 24 or 28.
    • Cellular localization

      Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
    • Target information above from: UniProt accession P15941 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4582 Human
      • Omim: 158340 Human
      • SwissProt: P15941 Human
      • Unigene: 89603 Human
      • Alternative names

        • ADMCKD antibody
        • ADMCKD1 antibody
        • Breast carcinoma associated antigen DF3 antibody
        • Breast carcinoma-associated antigen DF3 antibody
        • CA 15-3 antibody
        • CA15 3 antibody
        • CA15 3 antigen antibody
        • CA15-3 antibody
        • CA15.3 antibody
        • Cancer antigen 15-3 antibody
        • Carcinoma associated mucin antibody
        • Carcinoma-associated mucin antibody
        • CD 227 antibody
        • CD227 antibody
        • DF3 antigen antibody
        • EMA antibody
        • Episialin antibody
        • Epithelial Membrane Antigen antibody
        • H23 antigen antibody
        • H23AG antibody
        • KL 6 antibody
        • KL-6 antibody
        • KL6 antibody
        • Krebs von den Lungen-6 antibody
        • MAM 6 antibody
        • MAM6 antibody
        • MCD antibody
        • MCKD antibody
        • MCKD1 antibody
        • Medullary cystic kidney disease 1 (autosomal dominant) antibody
        • Medullary cystic kidney disease, autosomal dominant antibody
        • MUC 1 antibody
        • MUC-1 antibody
        • MUC-1/SEC antibody
        • MUC-1/X antibody
        • MUC1 antibody
        • MUC1-alpha antibody
        • MUC1-beta antibody
        • MUC1-CT antibody
        • MUC1-NT antibody
        • MUC1/ZD antibody
        • MUC1_HUMAN antibody
        • Mucin 1 antibody
        • Mucin 1 cell surface associated antibody
        • Mucin 1 transmembrane antibody
        • Mucin 1, cell surface associated antibody
        • Mucin-1 subunit beta antibody
        • Peanut reactive urinary mucin antibody
        • Peanut-reactive urinary mucin antibody
        • PEM antibody
        • PEMT antibody
        • Polymorphic epithelial mucin antibody
        • PUM antibody
        • Tumor associated epithelial membrane antigen antibody
        • Tumor associated epithelial mucin antibody
        • Tumor associated mucin antibody
        • Tumor-associated epithelial membrane antigen antibody
        • Tumor-associated mucin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [BC-2] (ab89492)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [BC-2] (ab89492)

        IHC image of MUC1 staining in human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89492, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (3)

      Publishing research using ab89492? Please let us know so that we can cite the reference in this datasheet.

      ab89492 has been referenced in 3 publications.

      • Radziejewska I  et al. Luteolin alters MUC1 extracellular domain, sT antigen, ADAM-17, IL-8, IL-10 and NF-?B expression in Helicobacter pylori-infected gastric cancer CRL-1739 cells: A preliminary study. Biomed Rep 14:19 (2021). PubMed: 33335725
      • Radziejewska I  et al. Lotus tetragonolobus and Maackia amurensis lectins influence phospho-I?Ba, IL-8, Lewis b and H type 1 glycoforms levels in H. pylori infected CRL-1739 gastric cancer cells. Adv Med Sci 63:205-211 (2018). PubMed: 29197783
      • Xing PX  et al. Monoclonal antibodies reactive with mucin expressed in breast cancer. Immunol Cell Biol 67 ( Pt 3):183-95 (1989). PubMed: 2477330

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Wants to use ab in IHC with tumour and non-tumour tissue. Wants to know if antibody is likely to detect glyclosylated and unglycos form. Also, check if we have the clone 214D4 with same question.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 05 2012

      Answer

      Thank you for contacting us last week in regards to antibodies raised against MUC1. I now have further information to share with you in regards to the mouse monoclonal antibody ab89492.

      The epitope recognised by the antibody is expected to be less sensitive to glycosylation than other epitopes. Please refer to the reference attached to this email.

      From this study it was concluded that the antibody produced from clone BC-2 reacted with both glycosylated and unglycosylated MUC1. However, this was only determined with O-linked carbohydrates, not with N-linked carbohydrates.

      In regards to the clone 214D4, we do not unfortunately have this available from our catalogue at present.

      I hope this information has been of help. If I can be of any further assistance, please do not hesitate to ask.

      Read More

      Abcam Scientific Support

      Answered on Nov 05 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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