• Product name

    Anti-MUC1 antibody [C595 (NCRC48)]
    See all MUC1 primary antibodies
  • Description

    Mouse monoclonal [C595 (NCRC48)] to MUC1
  • Host species

  • Specificity

    ab28081 recognises the breast cancer associated mucin encoded by the Muc-1 gene, CD227. In normal tissues expression is restricted to specialised glandular epithelial cells.
  • Tested applications

    Suitable for: Flow Cyt, ELISA, IHC-Fr, IHC-P, WB, ICC/IF, Othermore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Urinary MUC-1 mucin (Human)

  • Epitope

    ab28081 recognises the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin.
  • Positive control

    • Breast carcinoma



Our Abpromise guarantee covers the use of ab28081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

ELISA 1/100 - 1/1000.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
AP Use at an assay dependent concentration. PubMed: 21077635
ICC/IF Use at an assay dependent concentration.
Other Use at an assay dependent concentration. PubMed: 21077635


  • Function

    The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
    The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
  • Tissue specificity

    Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
  • Involvement in disease

    MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
    Medullary cystic kidney disease 1
  • Sequence similarities

    Contains 1 SEA domain.
  • Developmental stage

    During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
  • Post-translational

    Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
    Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
    Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
    Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
    The N-terminal sequence has been shown to begin at position 24 or 28.
  • Cellular localization

    Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADMCKD antibody
    • ADMCKD1 antibody
    • Breast carcinoma associated antigen DF3 antibody
    • Breast carcinoma-associated antigen DF3 antibody
    • CA 15-3 antibody
    • CA15 3 antibody
    • CA15 3 antigen antibody
    • CA15-3 antibody
    • CA15.3 antibody
    • Cancer antigen 15-3 antibody
    • Carcinoma associated mucin antibody
    • Carcinoma-associated mucin antibody
    • CD 227 antibody
    • CD227 antibody
    • DF3 antigen antibody
    • EMA antibody
    • Episialin antibody
    • Epithelial Membrane Antigen antibody
    • H23 antigen antibody
    • H23AG antibody
    • KL 6 antibody
    • KL-6 antibody
    • KL6 antibody
    • Krebs von den Lungen-6 antibody
    • MAM 6 antibody
    • MAM6 antibody
    • MCD antibody
    • MCKD antibody
    • MCKD1 antibody
    • Medullary cystic kidney disease 1 (autosomal dominant) antibody
    • Medullary cystic kidney disease, autosomal dominant antibody
    • MUC 1 antibody
    • MUC-1 antibody
    • MUC-1/SEC antibody
    • MUC-1/X antibody
    • MUC1 antibody
    • MUC1-alpha antibody
    • MUC1-beta antibody
    • MUC1-CT antibody
    • MUC1-NT antibody
    • MUC1/ZD antibody
    • MUC1_HUMAN antibody
    • Mucin 1 antibody
    • Mucin 1 cell surface associated antibody
    • Mucin 1 transmembrane antibody
    • Mucin 1, cell surface associated antibody
    • Mucin-1 subunit beta antibody
    • Peanut reactive urinary mucin antibody
    • Peanut-reactive urinary mucin antibody
    • PEM antibody
    • PEMT antibody
    • Polymorphic epithelial mucin antibody
    • PUM antibody
    • Tumor associated epithelial membrane antigen antibody
    • Tumor associated epithelial mucin antibody
    • Tumor associated mucin antibody
    • Tumor-associated epithelial membrane antigen antibody
    • Tumor-associated mucin antibody
    see all


  • Ab28081 staining human normal lung. Staining is localised to the apical cell membrane.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
  • Anti-MUC1 antibody [C595 (NCRC48)] (ab28081) + Whole cell lysate prepared from human BT20 cells

    Predicted band size: 122 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?

  • FITC-conjugated ab28081 staining MUC1 in MCF-7 cells (green). Nuclei are counterstained with DAPI (blue).


This product has been referenced in:

  • Pan MY  et al. Spectral contrast imaging method for mapping transmission surface plasmon images in metallic nanostructures. Biosens Bioelectron 142:111545 (2019). Read more (PubMed: 31376712) »
  • Stempin S  et al. Morphological and molecular characterization of the human breast epithelial cell line M13SV1 and its tumorigenic derivatives M13SV1-R2-2 and M13SV1-R2-N1. Cancer Cell Int 15:110 (2015). ICC/IF ; Human . Read more (PubMed: 26612978) »
See all 12 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


You are correct, the molecular weight of the core protein of MUC1 is calculated to be 120-225 kDa, while the mature glycosylated protein has a range of 250-500 kDa (Lancaster et al., 1990; Gendler et al., 1991; Gendler and Spicer, 1995).

We have 2 antibodies in the catalogue which may be of interest to you:

Anti-MUC1 antibody [C595 (NCRC48)] (ab28081)
Anti-MUC1 antibody (ab101352)

These are both guaranteed to work in western blot in human and detect a band at ˜170kDa.

Read More


Thank you for contacting us.

The epitope has only a 50% homology with the murine muc1. We therefore do not recommend this product for use in murine.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your inquiry.

Unfortunately, we do not offer a MUC1 fulllength protein. The MUC1 protein (ab80082) is only a recombinant fragment and all others are peptides. I am sorry for the inconvenience but I cannot recommend any of these as positive control for your experiment.

The immunogen for ab28081 washuman Urinary MUC-1 mucin.I am happy to confirm that the epitopethis clone ([C595 (NCRC48)])recognizes was mapped to the peptide epitope ARG-PRO-ALA-PRO within the protein core of the mucin. The epitope sequence RPAP is a repeated motif that occurs about 40 times along the length of the protein (between residues 126 to 965).

I am sorry that I could not answer all your questions on this occasion and hope this information was helpful nevertheless.

Read More


Thank you for your inquiry.

I can confirm that these two antibodies were not tested in sandwich ELISA yet. Unfortunately, we cannot give any discount. But there would be the possiblity to take part in our testing discount programm and to recieve a free antibody.

But I cannot recommend it in this case because we do not even know if ab109185 work in ELISA at all and we do have antibodies that are tested and guaranteed for sandwich ELISA in our catalogue:

ab10119 (Anti-MUC1 antibody [M3A106] (HRP)) can be be paired for ELISA with Mouse monoclonal [M10H6] to MUC1 (ab10117) and Mouse monoclonal [M4H2] to MUC1 (ab10120) and Mouse monoclonal [M8C9] to MUC1 (ab10123).

Please follow these links to the respective datasheets:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=10119).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=10117).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=10120).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=10123).

I hope this information is helpful. Please do not hesiate to conatc me with any further qestions.

Read More


Sorry for the delay in contacting you with regards to this query. We have a number of pairs of mouse monoclonals which can be used to detect the under or unglycosylated form of MUC1 (for example ab10117 which can be paired with HRP conjugated ab24471). However if you are interested in the glycosylated form of MUC1, unfortunately we have not tested any antibody pairs in order to detect this form and therefore cannot guarantee that it will work. However, form the pair which you presented, ab28081 has been tested in ELISA with human protein and should therefore be suitable for this application. However, I cannot be sure that the rabbit polyclonal ab15481 will work as it has not been tested in ELISA before and has only been used in applications where the immunogen may be in the denatured form. Two possible alternative would be ab79226 which recognises the Y1229 phosphorylated form (cytoplasmic) or ab14690 which again recognises the underglycosylated form (extracellular) but have both been tested using ELISA. Alternatively, ab80952 is an Armenian Hamster monoclonal reactive against the cytoplasmic tail of MUC1, or ab37435 which is a rabbit polyclonal, again directed against the cytoplasmic domain. Alternatively ab45167 is a rabbit monoclonal which recognises the extracellular domain but only of isoform 7 or MUC1. These have been used in flow cytometry and immunoprecipitation and are therefore more likely to be able to detect the native form of the protein. If you would like to test ab15481 as you suggested (or any of the other antibodies suggested which have not been used in ELISA yet), as it has not been used in ELISA before I can offer a testing discount. This would involve you purchasing the antibody, using it and letting us know the results via an Abreview (positive or negative), you would then be eligible for a free primary antibody of your choice from our catalog. More information on this offer can be found here: www.abcam.com/collaborationdiscount If you need a secondary antibody for this application we have a very easy to use search engine to allow you to find exactly what you are looking for: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 If you would like any further information, help in choosing a suitable secondary antibody or you are interested in applying for the testing discount please do not hesitate to contact me.

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