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  1. Link

    muc1-antibody-ep1024y-ab45167.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MUC1 antibody [EP1024Y] (ab45167)

  • Datasheet
  • SDS
Reviews (4)Q&A (3)References (25)

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Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
  • Anti-MUC1 antibody [EP1024Y] (ab45167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1024Y] to MUC1
  • Suitable for: WB, Flow Cyt, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-MUC1 antibody [EP1024Y]
    See all MUC1 primary antibodies
  • Description

    Rabbit monoclonal [EP1024Y] to MUC1
  • Host species

    Rabbit
  • Specificity

    Based on the immunogen sequence, the antibody recognises several isoforms of MUC1 (Uniprot ID P15941). They are Isoform Y (28 kDa), Isoform Y-LSP (28 kDa), Isoform S2 (17 kDa) and Isoform J13 (28 kDa).
  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MUC1 aa 1-100 (N terminal).

  • Positive control

    • WB: HeLa, T47D, MCF7 and A549 cell lysates; Human kidney, human breast carcinoma, human thyroid carcinoma and human colon cancer lysates; Human fetal lung lysate; Rat liver lysate and mouse liver lysate. ICC/IF: MCF7 cells. Flow Cyt: T47D and A549 cells.
  • General notes

    Isoform 7 of MUC1 behaves as a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling.

    The mouse and rat recommendation is based on WB results.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    Isoform 7 of MUC1 behaves as a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling.
  • Clonality

    Monoclonal
  • Clone number

    EP1024Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

Associated products

  • Alternative Versions

    • PE Anti-MUC1 antibody [EP1024Y] (ab213337)
    • Anti-MUC1 antibody [EP1024Y] - Low endotoxin, Azide free (ab218998)
    • Anti-MUC1 antibody [EP1024Y] - BSA and Azide free (ab256588)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human MUC1 knockout HeLa cell line (ab255412)
  • KO cell lysates

    • Human MUC1 knockout HeLa cell lysate (ab263764)
  • Positive Controls

    • T-47D whole cell lysate (ab14899)
    • Mouse liver tissue lysate - total protein (ab29301)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human MUC1 protein (ab80082)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 27 kDa.

MUC1 isoform 7.

Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/20.

For unpurified use at 1/50.

ICC/IF
1/500.
Notes
WB
1/1000. Detects a band of approximately 27 kDa.

MUC1 isoform 7.

Flow Cyt
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/20.

For unpurified use at 1/50.

ICC/IF
1/500.

Target

  • Function

    The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
    The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
  • Tissue specificity

    Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
  • Involvement in disease

    MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
    Medullary cystic kidney disease 1
  • Sequence similarities

    Contains 1 SEA domain.
  • Developmental stage

    During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
  • Post-translational
    modifications

    Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
    Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
    Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
    Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
    The N-terminal sequence has been shown to begin at position 24 or 28.
  • Cellular localization

    Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
  • Target information above from: UniProt accession P15941 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4582 Human
    • Entrez Gene: 17829 Mouse
    • Entrez Gene: 24571 Rat
    • Omim: 158340 Human
    • SwissProt: P15941 Human
    • SwissProt: Q02496 Mouse
    • Unigene: 89603 Human
    • Unigene: 16193 Mouse
    • Alternative names

      • ADMCKD antibody
      • ADMCKD1 antibody
      • Breast carcinoma associated antigen DF3 antibody
      • Breast carcinoma-associated antigen DF3 antibody
      • CA 15-3 antibody
      • CA15 3 antibody
      • CA15 3 antigen antibody
      • CA15-3 antibody
      • CA15.3 antibody
      • Cancer antigen 15-3 antibody
      • Carcinoma associated mucin antibody
      • Carcinoma-associated mucin antibody
      • CD 227 antibody
      • CD227 antibody
      • DF3 antigen antibody
      • EMA antibody
      • Episialin antibody
      • Epithelial Membrane Antigen antibody
      • H23 antigen antibody
      • H23AG antibody
      • KL 6 antibody
      • KL-6 antibody
      • KL6 antibody
      • Krebs von den Lungen-6 antibody
      • MAM 6 antibody
      • MAM6 antibody
      • MCD antibody
      • MCKD antibody
      • MCKD1 antibody
      • Medullary cystic kidney disease 1 (autosomal dominant) antibody
      • Medullary cystic kidney disease, autosomal dominant antibody
      • MUC 1 antibody
      • MUC-1 antibody
      • MUC-1/SEC antibody
      • MUC-1/X antibody
      • MUC1 antibody
      • MUC1-alpha antibody
      • MUC1-beta antibody
      • MUC1-CT antibody
      • MUC1-NT antibody
      • MUC1/ZD antibody
      • MUC1_HUMAN antibody
      • Mucin 1 antibody
      • Mucin 1 cell surface associated antibody
      • Mucin 1 transmembrane antibody
      • Mucin 1, cell surface associated antibody
      • Mucin-1 subunit beta antibody
      • Peanut reactive urinary mucin antibody
      • Peanut-reactive urinary mucin antibody
      • PEM antibody
      • PEMT antibody
      • Polymorphic epithelial mucin antibody
      • PUM antibody
      • Tumor associated epithelial membrane antigen antibody
      • Tumor associated epithelial mucin antibody
      • Tumor associated mucin antibody
      • Tumor-associated epithelial membrane antigen antibody
      • Tumor-associated mucin antibody
      see all

    Images

    • Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      All lanes : Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/1000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
      Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) cell lysate
      Lane 3 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 4 : MUC1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Observed band size: 24 kDa why is the actual band size different from the predicted?



      Lanes 1- 4: Merged signal (red and green). Green - ab45167 observed at 24 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab45167 was shown to react with MUC1 in Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) cells in western blot. Loss of signal was observed when knockout cell line ab255412 (knockout cell lysate ab263764) was used. Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) and MUC1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab45167 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [EP1024Y] (ab45167)

      Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling purified MUC1 with ab45167 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue). 

      Confocal image showing membranous staining on MCF7 cell line.

      Negative control: HCT-116 (PMID: 14998492).

    • Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (ab45167)

      Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) cells labelling MUC1 with unpurified ab45167 (pink) at a dilution of 1/150. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as the isotype control (green).

    • Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      All lanes : Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/1000 dilution (purified)

      Lane 1 : Human colon cancer lysate
      Lane 2 : Rat liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Observed band size: 27 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    • Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Immunoprecipitation - Anti-MUC1 antibody [EP1024Y] (ab45167)

      ab45167 (purified) at 1/20 dilution (2ug) immunoprecipitating MUC1 in Human fetal lung lysate.

      Lane 1 (input): Human fetal lung lysate 10ug

      Lane 2 (+): ab45167 + Human fetal lung lysate 10ug

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45167 in Human fetal lung lysate

      For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Flow Cytometry - Anti-MUC1 antibody [EP1024Y] (ab45167)

      Flow Cytometry analysis of A549 (human lung carcinoma cell line) cells labeling MUC1 with purified ab45167 at 1/20 dilution (10 ug/ml). Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution. Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabeled control, cells without incubation with primary antibody and secondary antibody.

    • Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/5000 dilution (purified) + Mouse liver lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Observed band size: 27 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Western blot - Anti-MUC1 antibody [EP1024Y] (ab45167)
      Anti-MUC1 antibody [EP1024Y] (ab45167) at 1/2000 dilution (unpurified) + T47D cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Observed band size: 27 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-MUC1 antibody [EP1024Y] (ab45167)
      Anti-MUC1 antibody [EP1024Y] (ab45167)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (25)

    Publishing research using ab45167? Please let us know so that we can cite the reference in this datasheet.

    ab45167 has been referenced in 25 publications.

    • Lv J  et al. Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages. Cell Discov 7:24 (2021). PubMed: 33850112
    • Tao S  et al. Methylation-Mediated Silencing of MicroRNA-497 Promotes Breast Cancer Progression Through Up-Regulation of Mucin1. Front Oncol 10:552099 (2020). PubMed: 33194611
    • Guo M  et al. MUC1 plays an essential role in tumor immunity of colorectal cancer stem cell vaccine. Int Immunopharmacol 85:106631 (2020). PubMed: 32470879
    • Liu X  et al. Nondestructive analysis of tumor-associated membrane protein MUC1 in living cells based on dual-terminal amplification of a DNA ternary complex. Theranostics 10:4410-4421 (2020). PubMed: 32292504
    • Eder N  et al. YAP1/TAZ drives ependymoma-like tumour formation in mice. Nat Commun 11:2380 (2020). PubMed: 32404936
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody [EP1024Y]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mammary gland in lactation)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    Mammary gland in lactation
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 08 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody [EP1024Y]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Kidney)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3%Tween in PBS
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Timo Rademakers

    Verified customer

    Submitted Oct 01 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MUC1 antibody [EP1024Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
    Permeabilization
    No
    Specification
    Prostate cancer
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-MUC1 antibody [EP1024Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E14.5 gut epithelium)
    Specification
    E14.5 gut epithelium
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 13 2012

    Question

    I have a quick question regarding your anti-MUC1 antibody [EP1024Y] (ab45167). I am not able to find any images of its use in combination with a fluorescent secondary antibody, do you know if it is suitable for this purpose?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 23 2012

    Answer

    Thank you for contacting us.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    As shown on the product datasheet, ab45167 is not suitable for ICC/IF but it is guaranteed for IHC-Fr, WB, IHC-P, Flow Cyt, IP and on Mouse, Rat, Human samples.

    Regarding the use of ab45167 in fluorescent detection method, we have an image for IHC-Fr where an Alexa Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary.

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 23 2012

    Question

    Hi, I was wondering if you could please send me what conditions were successful for this antibody for IHC paraffin embedded tissue? I saw 1:100 - 1:250 is the suggested dilution with antigen retrieval. Can you please tell me what percentage of block is recommended and for how long? Any information you can offer would be greatly appreciated.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2012

    Answer

    Thank you for contacting us.

    I have attached the protocol used by the lab for ab45167 anti-MUC1 antibody. The exact concentration that works best in your samples will have to be determined experimentally but we do recommend the starting range which you noted.I hope you find this information is helpful.

    Please do not hesitate to contact us if you need any more advice or information.

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    Abcam Scientific Support

    Answered on Apr 13 2012

    Question

    What is the immunogen sequence for ab45167 and does it map to the extracellular domain on the mouse MUC1?

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    Asked on Oct 20 2011

    Answer

    Thank you for contacting Abcam about ab45167. The immunogen for ab45167, does not map to the extracellular domain of mouse MUC1, as it does not share any sequence similarity with it. If there is anything else I can help you with, please do not hesitate to contac

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    Abcam Scientific Support

    Answered on Oct 20 2011

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