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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-MUC1 antibody [GP14 + E29] - BSA and Azide free (ab269802)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab269802)

    Key features and details

    • Mouse monoclonal [GP14 + E29] to MUC1 - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Human

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    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
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    Recombinant Human MUC1 protein (ab80082)

    View more associated products

    Overview

    • Product name

      Anti-MUC1 antibody [GP14 + E29] - BSA and Azide free
      See all MUC1 primary antibodies
    • Description

      Mouse monoclonal [GP14 + E29] to MUC1 - BSA and Azide free
    • Host species

      Mouse
    • Specificity

      Isotypes - IgG1, kappa (GP1.4) + IgG2a, kappa (E29).

    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human MUC1. Human milk fat globule membranes (GP1.4); Delipidated extract of Human milk fat globule membranes (E29).

    • Positive control

      • Human breast or colon carcinoma tissue.
    • General notes

      ab269802 is a carrier free version of ab200543. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes. Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Clonality

      Monoclonal
    • Clone number

      GP14 + E29
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • MUC
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MUC
      • Cancer
      • Tumor immunology
      • Tumor-associated antigens

    Associated products

    • Alternative Versions

      • Anti-MUC1 antibody [GP14 + E29] (ab200543)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Recombinant Protein

      • Recombinant Human MUC1 protein (ab80082)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab269802 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 0.5 - 1 µg/ml.
    Notes
    IHC-P
    Use a concentration of 0.5 - 1 µg/ml.

    Target

    • Function

      The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
      The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
    • Tissue specificity

      Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
    • Involvement in disease

      MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
      Medullary cystic kidney disease 1
    • Sequence similarities

      Contains 1 SEA domain.
    • Developmental stage

      During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
    • Post-translational
      modifications

      Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
      Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
      Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
      Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
      The N-terminal sequence has been shown to begin at position 24 or 28.
    • Cellular localization

      Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
    • Target information above from: UniProt accession P15941 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4582 Human
      • Omim: 158340 Human
      • SwissProt: P15941 Human
      • Unigene: 89603 Human
      • Alternative names

        • ADMCKD antibody
        • ADMCKD1 antibody
        • Breast carcinoma associated antigen DF3 antibody
        • Breast carcinoma-associated antigen DF3 antibody
        • CA 15-3 antibody
        • CA15 3 antibody
        • CA15 3 antigen antibody
        • CA15-3 antibody
        • CA15.3 antibody
        • Cancer antigen 15-3 antibody
        • Carcinoma associated mucin antibody
        • Carcinoma-associated mucin antibody
        • CD 227 antibody
        • CD227 antibody
        • DF3 antigen antibody
        • EMA antibody
        • Episialin antibody
        • Epithelial Membrane Antigen antibody
        • H23 antigen antibody
        • H23AG antibody
        • KL 6 antibody
        • KL-6 antibody
        • KL6 antibody
        • Krebs von den Lungen-6 antibody
        • MAM 6 antibody
        • MAM6 antibody
        • MCD antibody
        • MCKD antibody
        • MCKD1 antibody
        • Medullary cystic kidney disease 1 (autosomal dominant) antibody
        • Medullary cystic kidney disease, autosomal dominant antibody
        • MUC 1 antibody
        • MUC-1 antibody
        • MUC-1/SEC antibody
        • MUC-1/X antibody
        • MUC1 antibody
        • MUC1-alpha antibody
        • MUC1-beta antibody
        • MUC1-CT antibody
        • MUC1-NT antibody
        • MUC1/ZD antibody
        • MUC1_HUMAN antibody
        • Mucin 1 antibody
        • Mucin 1 cell surface associated antibody
        • Mucin 1 transmembrane antibody
        • Mucin 1, cell surface associated antibody
        • Mucin-1 subunit beta antibody
        • Peanut reactive urinary mucin antibody
        • Peanut-reactive urinary mucin antibody
        • PEM antibody
        • PEMT antibody
        • Polymorphic epithelial mucin antibody
        • PUM antibody
        • Tumor associated epithelial membrane antigen antibody
        • Tumor associated epithelial mucin antibody
        • Tumor associated mucin antibody
        • Tumor-associated epithelial membrane antigen antibody
        • Tumor-associated mucin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab269802)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody (ab269802)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling MUC1 with ab200543 at 1 µg/mL.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200543).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab269802? Please let us know so that we can cite the reference in this datasheet.

      ab269802 has not yet been referenced specifically in any publications.

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