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Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)

  • Datasheet
Reviews (1)Q&A (6)References (15)

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Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
  • Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
  • Western blot - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
  • Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)

Key features and details

  • Mouse monoclonal [HMFG1 (aka 1.10.F3)] to MUC1
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]
    See all MUC1 primary antibodies
  • Description

    Mouse monoclonal [HMFG1 (aka 1.10.F3)] to MUC1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human MUC1. Delipidated human milk fat globule membrane.

  • Epitope

    This antibody recognizes a peptide epitope (PDTR) within the VNTR region of the extracellular domain of MUC1 (PubMed ID: PMC3021526).
  • Positive control

    • IHC-P: Human normal colon FFPE tissue sections. ICC: MCF7 cells. WB: Human breast tissue lysate, human colon tissue lysate and MCF whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    For a more comprehensive guide to this epitope of HMFG1 clone, we recommend the following publications;

    Petrakou E et al. 1998: Epitope Mapping of Anti-MUC1 Mucin Protein Core Monoclonal Antibodies. Tumour Biology.

    Verhoeyen ME et al. 1993: Construction of a reshaped HMFG1 antibody and comparison of its fine specificity with that of the parent mouse antibody. Immunology.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    HMFG1 (aka 1.10.F3)
  • Myeloma

    P3-NS1/1-Ag4-1
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens

Associated products

  • Alternative Versions

    • PE Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab215670)
    • HRP Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab215778)
    • Alexa Fluor® 488 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab223133)
    • Alexa Fluor® 647 Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab223134)
    • Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] - BSA and Azide free (ab230298)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human MUC1 protein (ab80082)

Applications

Our Abpromise guarantee covers the use of ab70475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
IHC-P Use at an assay dependent concentration.
ICC Use a concentration of 10 µg/ml.

Target

  • Function

    The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
    The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
  • Tissue specificity

    Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
  • Involvement in disease

    MUC1/CA 15-3 is used as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence (PubMed:20816948). Decreased levels over time may be indicative of a positive response to treatment. Conversely, increased levels may indicate disease progression. At an early stage disease, only 21% of patients exhibit high MUC1/CA 15-3 levels, that is why CA 15-3 is not a useful screening test. Most antibodies target the highly immunodominant core peptide domain of 20 amino acid (APDTRPAPGSTAPPAHGVTS) tandem repeats. Some antibodies recognize glycosylated epitopes.
    Medullary cystic kidney disease 1
  • Sequence similarities

    Contains 1 SEA domain.
  • Developmental stage

    During fetal development, expressed at low levels in the colonic epithelium from 13 weeks of gestation.
  • Post-translational
    modifications

    Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM.
    Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17.
    Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane.
    Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1.
    The N-terminal sequence has been shown to begin at position 24 or 28.
  • Cellular localization

    Secreted; Cell membrane. Cytoplasm. Nucleus. On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus and Apical cell membrane. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions.
  • Target information above from: UniProt accession P15941 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4582 Human
    • Omim: 158340 Human
    • SwissProt: P15941 Human
    • Unigene: 89603 Human
    • Alternative names

      • ADMCKD antibody
      • ADMCKD1 antibody
      • Breast carcinoma associated antigen DF3 antibody
      • Breast carcinoma-associated antigen DF3 antibody
      • CA 15-3 antibody
      • CA15 3 antibody
      • CA15 3 antigen antibody
      • CA15-3 antibody
      • CA15.3 antibody
      • Cancer antigen 15-3 antibody
      • Carcinoma associated mucin antibody
      • Carcinoma-associated mucin antibody
      • CD 227 antibody
      • CD227 antibody
      • DF3 antigen antibody
      • EMA antibody
      • Episialin antibody
      • Epithelial Membrane Antigen antibody
      • H23 antigen antibody
      • H23AG antibody
      • KL 6 antibody
      • KL-6 antibody
      • KL6 antibody
      • Krebs von den Lungen-6 antibody
      • MAM 6 antibody
      • MAM6 antibody
      • MCD antibody
      • MCKD antibody
      • MCKD1 antibody
      • Medullary cystic kidney disease 1 (autosomal dominant) antibody
      • Medullary cystic kidney disease, autosomal dominant antibody
      • MUC 1 antibody
      • MUC-1 antibody
      • MUC-1/SEC antibody
      • MUC-1/X antibody
      • MUC1 antibody
      • MUC1-alpha antibody
      • MUC1-beta antibody
      • MUC1-CT antibody
      • MUC1-NT antibody
      • MUC1/ZD antibody
      • MUC1_HUMAN antibody
      • Mucin 1 antibody
      • Mucin 1 cell surface associated antibody
      • Mucin 1 transmembrane antibody
      • Mucin 1, cell surface associated antibody
      • Mucin-1 subunit beta antibody
      • Peanut reactive urinary mucin antibody
      • Peanut-reactive urinary mucin antibody
      • PEM antibody
      • PEMT antibody
      • Polymorphic epithelial mucin antibody
      • PUM antibody
      • Tumor associated epithelial membrane antigen antibody
      • Tumor associated epithelial mucin antibody
      • Tumor associated mucin antibody
      • Tumor-associated epithelial membrane antigen antibody
      • Tumor-associated mucin antibody
      see all

    Images

    • Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)Lab

      ab70475 staining MUC1 in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab70475 at 10µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)

      IHC image of MUC1 staining in human normal colon formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70475, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      Ab70475 staining human normal lung. Staining is localised to the apical membrane.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)

      ab70475 stained MCF7 cells. The cells were 4% formaldehyde fixed for 10 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70475 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1hour at room temperature. ab195889 Anti-alpha Tubulin (Alexa Fluor® 594) was used as a counterstaining (pseudo-colored red) at a 1/250 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Western blot - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      Western blot - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      All lanes : Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475) at 1 µg/ml

      Lane 1 : Human breast tissue lysate - total protein (ab30090)
      Lane 2 : Human colon tissue lysate - total protein (ab30051)
      Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 122 kDa


      Exposure time: 4 minutes
    • Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)
      Immunocytochemistry - Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475)Image courtesy of an anonymous Abreview.

      ab70475 staining MUC1 in human breast cancer cell line T47D by Immunocytochemistry. Cells were fixed in methanol, blocked using 10% serum for 5 minutes at 25°C and then incubated with ab70475 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse polyclonal.

      See Abreview

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (15)

    Publishing research using ab70475? Please let us know so that we can cite the reference in this datasheet.

    ab70475 has been referenced in 15 publications.

    • Rojas-Sepúlveda D  et al. Tumor lysate-based vaccines: on the road to immunotherapy for gallbladder cancer. Cancer Immunol Immunother 67:1897-1910 (2018). PubMed: 29600445
    • Wang T  et al. Increasing circulating exosomes-carrying TRPC5 predicts chemoresistance in metastatic breast cancer patients. Cancer Sci 108:448-454 (2017). PubMed: 28032400
    • Abey SK  et al. Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress. BBA Clin 7:23-35 (2017). PubMed: 28053879
    • Chen Y  et al. Breast cancer resistance protein (BCRP)-containing circulating microvesicles contribute to chemoresistance in breast cancer. Oncol Lett 10:3742-3748 (2015). PubMed: 26788201
    • Lim ML  et al. Characterization of stem-like cells in mucoepidermoid tracheal paediatric tumor. PLoS One 9:e107712 (2014). ICC/IF ; Human . PubMed: 25229469
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Question

    Do you know what the epitope region of this antibody is?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 08 2012

    Answer

    Thank you for contacting Abcam.

    Delipidated human milk fat globule was used as the immunogen for ab70475 and unfortunately the exact epitope region for ab70475 has not been mapped.

    I am sorry that I could not help further in this matter.

    Read More

    Abcam Scientific Support

    Answered on Feb 08 2012

    Question

    Regarding the product Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] (ab70475). I read in multiple resources some conflict information about its reactivity with glycosylated MUC1 protein core. Some sources mentioned that it is glycosylation sensitive, while others said it's not. Could you please confirm that? I'm planning to use a type-O-glycosylation inhibitor prior to preparing the cell lysates for western blot. Is that gonna affect the binding of the antibody at all?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2013

    Answer

    We do not have in-house data for this clone other than the IHC and flow cytometry data, and it does not clarify whether glycosylation is required for reactivity. The older papers listed on the datasheet describe production and characterization, in particular the Taylor-Papadimitriou paper and the Burchell papers. I do not have access to the full articles though, so I do not know whether the antibody was tested for relative reactivity with glycosylated and de-glycosylated/non-glycosylated MUC1.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Oct 23 2013

    Question

    Customer kindly contacted us regarding the dilution of ab70475 and ab99632 for western blot, as well as our detection substrates for detecting HRP.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 26 2012

    Answer


    As we discussed, for purified primary antibodies such as ab70475 we recommend a starting dilution of 1ug/ml for western blot. For the secondary antibody ab99632, we were able to see a band in western blot from a dilution range of 1:2,000 to 1:10,000, with the band becoming increasingly fainter at increased dilutions.

    Additionally, I have included links to 2 of our ECL substrate kits for detecting HRP via western blot below:
    1. ab65623: https://www.abcam.com/ECL-Western-Blotting-Substrate-Kit-50-Tests-ab65623.html
    2. ab133409: https://www.abcam.com/Optiblot-ECL-Ultra-Detect-Kit-12pg-2ng-ab133409.html

    Read More

    Abcam Scientific Support

    Answered on Dec 26 2012

    Question

    I had a question about your antibody https://www.abcam.com/MUC1-antibody-HMFG1-aka-1-10-F3-ab70475.html. Its clone HMFG1. My question is this antibody cross-reactive with other mucins, MUC16 in particular. I did flow cytometry on MUC16 rich OVCAR-3 cells with MUC1(HMFG1, ab70475) and MUC16(X75) and both stained very well. Then I tried two other MUC1 antibodies(ab108185-EPR1023 and cell signaling VU4H5) and there was no staining. I'm just wondering if the HMFG1 antibody is a really good or if it is just cross-reactive staining.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 16 2012

    Answer

    Thank you for contacting us.

    We haven't tested this antibody for its cross-reactivity with MUC16. I BLASTed the MUC1 and MUC16 protein sequences, and there really isn't that much overlap. The greatest homology was around 20% and we generally say that >85% is necessary for cross-reactivity.

    Unfortunately I can't tell you definitively if the antibody is cross-reacting or not. The only real way to test it would be with a MUC16 recombinant protein, but unfortunately we do not carry any.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 16 2012

    Question

    I would like to know if the light chain typefor these antibodies has been determined?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 07 2012

    Answer

    The light chain type for ab70475 has not yet been determined.

    I am sorry I could not be more helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 07 2012

    Question

    I would like to know if the light chain typefor these antibodies has been determined?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 05 2012

    Answer

    I am sorry it is taking so long to reply to your enquiry. I am having trouble getting a response from the supplying lab. I will forward you any information I receive as soon as possible but as of now I do not know when I will have further details.

    I apologize for the inconvenience.

    Read More

    Abcam Scientific Support

    Answered on Jun 05 2012

    Immunocytochemistry abreview for Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Breast cancer cell line T47D)
    Specification
    Breast cancer cell line T47D
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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