Key features and details
- Rabbit polyclonal to MUC13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MUC13 antibody
See all MUC13 primary antibodies
DescriptionRabbit polyclonal to MUC13
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
A synthetic peptide from the C terminus of Human MUC13.
- Human colon tissue; 293, HeLa and COLO205 cell lysates; HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol, 49% PBS
PBS (without Mg2+, Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1999. Predicted molecular weight: 55 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionEpithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
Tissue specificityHighly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.
Sequence similaritiesContains 3 EGF-like domains.
Contains 1 SEA domain.
modificationsCleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
Cellular localizationCell membrane. Secreted. Also exists as a soluble form.
- Information by UniProt
- Down-regulated in colon cancer 1 antibody
- DRCC1 antibody
- FLJ20063 antibody
All lanes : Anti-MUC13 antibody (ab124654) at 1/500 dilution
Lane 1 : 293 cell lysate
Lane 2 : COLO205 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HeLa cell lysate with synthesized peptide
Predicted band size: 55 kDa
ab124654 staining MUC13 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked for 30 minutes at room temperature. Samples were incubated with primary antibody (1/25 in PBS Tween) for 1 hour. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab124654, at 5 µg/ml, staining MUC13 in Formalin-fixed, Paraffin-embedded Human Colon tissue by Immunohistochemistry.
ab124654 at 1/100 dilution staining MUC13 in HepG2 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
ab124654 has been referenced in 2 publications.
- Lin Q et al. Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H. Int J Oncol 53:266-274 (2018). PubMed: 29749468
- He L et al. Reduction of miR-132-3p contributes to gastric cancer proliferation by targeting MUC13. Mol Med Rep 15:3055-3061 (2017). WB, IHC-P ; Human . PubMed: 28339011