Product nameAnti-MUC13 antibody
See all MUC13 primary antibodies
DescriptionRabbit polyclonal to MUC13
Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide (Human) from an internal sequence.
- WB: Extracts from COLO, 293 and HeLa cells. IHC-P: Human colon carcinoma tissue. IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab65109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
|ICC/IF||1/500 - 1/1000.|
FunctionEpithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.
Tissue specificityHighly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.
Sequence similaritiesContains 3 EGF-like domains.
Contains 1 SEA domain.
modificationsCleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
Cellular localizationCell membrane. Secreted. Also exists as a soluble form.
- Information by UniProt
- Down-regulated in colon cancer 1 antibody
- DRCC1 antibody
- FLJ20063 antibody
All lanes : Anti-MUC13 antibody (ab65109) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from 293 cells
Lane 3 : extracts from HeLa cells
Lane 4 : extracts from HeLa cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
ICC/IF image of ab65109 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- LaMonte GM et al. Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection. Nat Commun 10:488 (2019). Read more (PubMed: 30700707) »
- Journo G et al. Modulation of Cellular CpG DNA Methylation by Kaposi's Sarcoma-Associated Herpesvirus. J Virol 92:N/A (2018). Read more (PubMed: 29899086) »