Anti-MUC13 antibody [C18] - BSA and Azide free (ab269565)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [C18] - BSA and Azide free (ab269565)](https://www.abcam.com/ps/products/269/ab269565/Images/ab269565-368324-AntiMUC13-antibody-C18-BSA-Azide-free-immunohistochemistry-colon-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [C18] - BSA and Azide free (ab269565)")
Key features and details
- Mouse monoclonal [C18] to MUC13 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-MUC13 antibody [C18] - BSA and Azide free
See all MUC13 primary antibodies -
Description
Mouse monoclonal [C18] to MUC13 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Recombinant fragment corresponding to Human MUC13.
Database link: Q9H3R2 -
Positive control
- ICC/IF: HT-29 cells IHC-P: FFPE normal human colon tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab269565 is the carrier-free version of ab231158. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Puriifed from TCS. -
Clonality
Monoclonal -
Clone number
C18 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. -
Tissue specificity
Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach. -
Sequence similarities
Contains 3 EGF-like domains.
Contains 1 SEA domain. -
Post-translational
modificationsCleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
Highly N-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble form. - Information by UniProt
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Database links
- Entrez Gene: 56667 Human
- Omim: 612181 Human
- SwissProt: Q9H3R2 Human
- Unigene: 5940 Human
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Alternative names
- Down-regulated in colon cancer 1 antibody
- DRCC1 antibody
- FLJ20063 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [C18] - BSA and Azide free (ab269565)
IHC image of MUC13 staining in a section of formalin-fixed paraffin-embedded normal human colon* performed on a Leica BONDTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab231158, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This image was produced using the same antibody clone but in a different formulation ab231158, PBS and sodium azide.
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![Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C18] - BSA and Azide free (ab269565)](https://www.abcam.com/ps/products/269/ab269565/Images/ab269565-368325-AntiMUC13-antibody-C18-BSA-Azide-free-immunocytochemistry-ht29-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [C18] - BSA and Azide free (ab269565)ab231158 staining MUC13 in HT-29 (Human colorectal adenocarcinoma cell line) cells.
The cells were fixed with 100% methanol (5 min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab231158 at 5 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red).
Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This image was produced using the same antibody clone but in a different formulation ab231158, PBS and sodium azide.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab269565 has not yet been referenced specifically in any publications.