Recombinant
RabMAb

Recombinant Anti-MUC13 antibody [EPR21901] (ab235450)

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Overview

  • Product name

    Anti-MUC13 antibody [EPR21901]
    See all MUC13 primary antibodies

  • Description

    Rabbit monoclonal [EPR21901] to MUC13

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MUC13 aa 400-500. The exact sequence is proprietary.
    Database link: Q9H3R2

  • Positive control

    • WB: HT-29, SW480, HCT 116 and LoVo whole cell lysates. IHC-P: Human colon, colorectal cancer and gastric cancer tissue. ICC/IF: LoVo and HT-29 cells. FC: LoVo cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab235450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 120, 80, 35 kDa (predicted molecular weight: 55 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/50.
Flow Cyt 1/40.

Target

  • Function

    Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.

  • Tissue specificity

    Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.

  • Sequence similarities

    Contains 3 EGF-like domains.
    Contains 1 SEA domain.

  • Post-translational
    modifications

    Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
    Highly N-glycosylated.

  • Cellular localization

    Cell membrane. Secreted. Also exists as a soluble form.
  • Information by UniProt

  • Database links

    • Alternative names

      • Down-regulated in colon cancer 1 antibody
      • DRCC1 antibody
      • FLJ20063 antibody
      • MUC 13 antibody
      • MUC-13 antibody
      • Muc13 antibody
      • MUC13 Down regulated in colon cancer 1 antibody
      • MUC13_HUMAN antibody
      • Mucin 13, cell surface associated antibody
      • Mucin-13 antibody
      • PRO20221 antibody
      • RECC antibody
      • UNQ6194 antibody
      see all

    Images

    • Western blot - Anti-MUC13 antibody [EPR21901] (ab235450)
      Western blot - Anti-MUC13 antibody [EPR21901] (ab235450)
      All lanes : Anti-MUC13 antibody [EPR21901] (ab235450) at 1/1000 dilution

      Lane 1 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
      Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
      Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
      Lane 4 : Human ovary cancer lysate at 10 µg
      Lane 5 : LoVo (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

      Secondary
      Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

      Predicted band size: 55 kDa
      Observed band size: 120,35,80 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lanes 1-3 and 5: 3 minutes, Lane 4: 26 seconds.

      The expression profile observed is consistent with what has been described in the literature (PMID: 15904467). Lane 3 was developed using a high sensitivity ECL substrate.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in epithelial cells of the normal human colon (PMID: 17360025) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)
      Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry - Anti-MUC13 antibody [EPR21901] (ab235450)
      Flow Cytometry - Anti-MUC13 antibody [EPR21901] (ab235450)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/40 (red) compared with a Isotype control details (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

      Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human gastric cancer (PMID: 15904467) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

      Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)

      Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the apical surface and in the cytoplasm in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)
      Immunocytochemistry/ Immunofluorescence - Anti-MUC13 antibody [EPR21901] (ab235450)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the LoVo cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    References

    ab235450 has not yet been referenced specifically in any publications.

    Publishing research using ab235450? Please let us know so that we can cite the reference in this datasheet.

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