Recombinant Anti-MUC13 antibody [EPR21901] (ab235450)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21901] to MUC13
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MUC13 antibody [EPR21901]
See all MUC13 primary antibodies -
Description
Rabbit monoclonal [EPR21901] to MUC13 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HT-29, SW480, HCT 116 and LoVo whole cell lysates. IHC-P: Human colon, colorectal cancer and gastric cancer tissue. ICC/IF: LoVo and HT-29 cells. Flow Cyt: LoVo cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21901 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
|
ICC/IF |
Human
|
IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 120, 80, 35 kDa (predicted molecular weight: 55 kDa).
|
|
IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/50.
|
|
Flow Cyt |
1/40.
|
Notes |
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WB
1/1000. Detects a band of approximately 120, 80, 35 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50. |
Flow Cyt
1/40. |
Target
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Function
Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. -
Tissue specificity
Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach. -
Sequence similarities
Contains 3 EGF-like domains.
Contains 1 SEA domain. -
Post-translational
modificationsCleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homo-oligomerization into dimers is dependent on intrachain disulfide bonds.
Highly N-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble form. - Information by UniProt
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Database links
- Entrez Gene: 56667 Human
- Omim: 612181 Human
- SwissProt: Q9H3R2 Human
- Unigene: 5940 Human
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Alternative names
- Down-regulated in colon cancer 1 antibody
- DRCC1 antibody
- FLJ20063 antibody
see all
Images
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All lanes : Anti-MUC13 antibody [EPR21901] (ab235450) at 1/1000 dilution
Lane 1 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 4 : Human ovary cancer lysate at 10 µg
Lane 5 : LoVo (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Predicted band size: 55 kDa
Observed band size: 120,35,80 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3 and 5: 3 minutes, Lane 4: 26 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 15904467). Lane 3 was developed using a high sensitivity ECL substrate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in epithelial cells of the normal human colon (PMID: 17360025) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/40 (red) compared with a Isotype control details (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human gastric cancer (PMID: 15904467) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive cytoplasmic staining in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC13 antibody [EPR21901] (ab235450)
Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue labeling MUC13 with ab235450 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the apical surface and in the cytoplasm in human colorectal cancer (PMID: 17360025) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LoVo (human colorectal adenocarcinoma cell line) cells labeling MUC13 with ab235450 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the LoVo cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (1)
ab235450 has been referenced in 1 publication.
- Ni Z et al. TGR5-HNF4a axis contributes to bile acid-induced gastric intestinal metaplasia markers expression. Cell Death Discov 6:56 (2020). PubMed: 32655894