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RecombinantRabMAb

Recombinant Anti-MUC16 antibody [EPSISR23-96] (ab168360)

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Western blot - Anti-MUC16 antibody [EPSISR23-96] (ab168360)
  • Anti-MUC16 antibody [EPSISR23-96] (ab168360)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPSISR23-96] to MUC16
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human MUC16 protein (ab164957)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-MUC16 antibody [EPSISR23-96]
    See all MUC16 primary antibodies
  • Description

    Rabbit monoclonal [EPSISR23-96] to MUC16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MUC16 aa 12450-12550.
    Database link: Q8WX17

  • Positive control

    • NIH:OVCAR-3, Human ovary cancer and Human uterus lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPSISR23-96
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • MUC
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • MUC

Associated products

  • Alternative Versions

    • Anti-MUC16 antibody [EPSISR23-96] - BSA and Azide free (ab249456)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MUC16 protein (ab164957)

Applications

Our Abpromise guarantee covers the use of ab168360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 1519 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • Tissue specificity

      Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • Sequence similarities

      Contains 2 ANK repeats.
      Contains 56 SEA domains.
    • Domain

      Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • Post-translational
      modifications

      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • Cellular localization

      Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Target information above from: UniProt accession Q8WXI7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 94025 Human
      • Omim: 606154 Human
      • SwissProt: Q8WXI7 Human
      • Unigene: 432676 Human
      • Alternative names

        • CA 125 antibody
        • CA-125 antibody
        • CA125 antibody
        • CA125 ovarian cancer antigen antibody
        • Cancer antigen 125 antibody
        • FLJ14303 antibody
        • MUC 16 antibody
        • MUC-16 antibody
        • MUC16 antibody
        • MUC16_HUMAN antibody
        • Mucin 16 antibody
        • Mucin 16 cell surface associated antibody
        • Mucin-16 antibody
        • Mucin16 antibody
        • Ovarian cancer related tumor marker CA125 antibody
        • Ovarian cancer-related tumor marker CA125 antibody
        • Ovarian carcinoma antigen CA125 antibody
        see all

      Images

      • Western blot - Anti-MUC16 antibody [EPSISR23-96] (ab168360)
        Western blot - Anti-MUC16 antibody [EPSISR23-96] (ab168360)
        All lanes : Anti-MUC16 antibody [EPSISR23-96] (ab168360) at 1/1000 dilution

        Lane 1 : NIH:OVCAR-3 lysate
        Lane 2 : Human ovary cancer lysate
        Lane 3 : Human uterus lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 1519 kDa

      • Anti-MUC16 antibody [EPSISR23-96] (ab168360)
        Anti-MUC16 antibody [EPSISR23-96] (ab168360)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab168360? Please let us know so that we can cite the reference in this datasheet.

      ab168360 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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