Recombinant Anti-MUC16 antibody [EPSISR23-96] (ab168360)
![Western blot - Anti-MUC16 antibody [EPSISR23-96] (ab168360)](https://www.abcam.com/ps/products/168/ab168360/Images/ab168360-166640-anti-muc16-antibody-epsisr23-96-western-blot.jpg "Western blot - Anti-MUC16 antibody [EPSISR23-96] (ab168360)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR23-96] to MUC16
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-MUC16 antibody [EPSISR23-96]
See all MUC16 primary antibodies -
Description
Rabbit monoclonal [EPSISR23-96] to MUC16 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MUC16 aa 12450-12550.
Database link: Q8WX17 -
Positive control
- NIH:OVCAR-3, Human ovary cancer and Human uterus lysates.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPSISR23-96 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 1519 kDa. |
Target
-
Function
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. -
Tissue specificity
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. -
Sequence similarities
Contains 2 ANK repeats.
Contains 56 SEA domains. -
Domain
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail. -
Post-translational
modificationsHeavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
Cellular localization
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. - Information by UniProt
-
Database links
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
-
Alternative names
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
Images
-
Western blot - Anti-MUC16 antibody [EPSISR23-96] (ab168360)All lanes : Anti-MUC16 antibody [EPSISR23-96] (ab168360) at 1/1000 dilution
Lane 1 : NIH:OVCAR-3 lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : Human uterus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 1519 kDa -
![Anti-MUC16 antibody [EPSISR23-96] (ab168360)](https://www.abcam.com/ps/products/168/ab168360/Images/ab168360-2-benefits-of-recombinant-antibodies.png)
Anti-MUC16 antibody [EPSISR23-96] (ab168360)
Datasheets and documents
References (0)
ab168360 has not yet been referenced specifically in any publications.
