RecombinantRabMAb

Recombinant Anti-MUC16 antibody [EPSISR23] (ab134093)

Submit a review Q&A (5)References (3)
Flow Cytometry - Anti-MUC16 antibody [EPSISR23] (ab134093)
  • Immunocytochemistry - Anti-MUC16 antibody [EPSISR23] (ab134093)
  • Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093)
  • Anti-MUC16 antibody [EPSISR23] (ab134093)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPSISR23] to MUC16
  • Suitable for: ICC, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

APC Carrier Free PE PerCP

Overview

  • Product name

    Anti-MUC16 antibody [EPSISR23]
    See all MUC16 primary antibodies

  • Description

    Rabbit monoclonal [EPSISR23] to MUC16

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human MUC16 aa 12450-12550.
    Database link: Q8WX17
    (Peptide available as ab139976)

  • Positive control

    • ICC: NIH:OVCAR-3 cells. Flow Cyt: HeLa cells. WB: NIH:OVCAR-3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/50.

For unpurified use at 1/100 - 1/250 dilution.
WB
1/10000. Detects a band of approximately 2353 kDa (predicted molecular weight: 1519 kDa).Can be blocked with MUC16 peptide (ab139976).

For unpurified use at 1/10000 - 1/50000 dilution.
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100 - 1/1000 dilution.
Notes
ICC
1/50.

For unpurified use at 1/100 - 1/250 dilution.
WB
1/10000. Detects a band of approximately 2353 kDa (predicted molecular weight: 1519 kDa).Can be blocked with MUC16 peptide (ab139976).

For unpurified use at 1/10000 - 1/50000 dilution.
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100 - 1/1000 dilution.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

  • Tissue specificity

    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.

  • Sequence similarities

    Contains 2 ANK repeats.
    Contains 56 SEA domains.

  • Domain

    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.

  • Post-translational
    modifications

    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

  • Cellular localization

    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • CA 125 antibody
      • CA-125 antibody
      • CA125 antibody
      • CA125 ovarian cancer antigen antibody
      • Cancer antigen 125 antibody
      • FLJ14303 antibody
      • MUC 16 antibody
      • MUC-16 antibody
      • MUC16 antibody
      • MUC16_HUMAN antibody
      • Mucin 16 antibody
      • Mucin 16 cell surface associated antibody
      • Mucin-16 antibody
      • Mucin16 antibody
      • Ovarian cancer related tumor marker CA125 antibody
      • Ovarian cancer-related tumor marker CA125 antibody
      • Ovarian carcinoma antigen CA125 antibody
      see all

    Images

    • Flow Cytometry - Anti-MUC16 antibody [EPSISR23] (ab134093)
      Flow Cytometry - Anti-MUC16 antibody [EPSISR23] (ab134093)
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling MUC16 with Purified ab134093 at 1:20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunocytochemistry - Anti-MUC16 antibody [EPSISR23] (ab134093)
      Immunocytochemistry - Anti-MUC16 antibody [EPSISR23] (ab134093)
      Immunocytochemistry analysis of NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labeling MUC16 with Purified ab134093 at 1:50 dilution (2.3 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093)
      Western blot - Anti-MUC16 antibody [EPSISR23] (ab134093)
      Anti-MUC16 antibody [EPSISR23] (ab134093) at 1/10000 dilution + NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 1519 kDa
      Observed band size: 2353 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Image produced using the purified version.

    • Anti-MUC16 antibody [EPSISR23] (ab134093)
      Anti-MUC16 antibody [EPSISR23] (ab134093)

    References (3)

    Publishing research using ab134093? Please let us know so that we can cite the reference in this datasheet.

    ab134093 has been referenced in 3 publications.

    • Fan Z  et al. Smartphone Biosensor System with Multi-Testing Unit Based on Localized Surface Plasmon Resonance Integrated with Microfluidics Chip. Sensors (Basel) 20:N/A (2020). PubMed: 31941128
    • O'Callaghan AR  et al. Oral Mucosa Tissue Equivalents for the Treatment of Limbal Stem Cell Deficiency. Adv Biosyst 4:e1900265 (2020). PubMed: 32515079
    • O'Callaghan AR  et al. Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use. Regen Med 11:529-43 (2016). PubMed: 27513189

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Question

    I just ordered anti-MUC16 antibody [EPSISR23]. I am wondering where is the epitope for this antibody, extracellular or intracellular? I found the immunogen of anti-MUC16 antibody is a synthetic peptides(ab 139976); however, I did not find the localization of the peptides on MUC16 protein (a transmembrane protein).

    Read More
    Answer

    The immunogen is extracellular.

    Read More

    Question

    He needs this produkt to storting with FACS and he has to do life staining.

    He need this cells to grow

    Read More
    Answer

    Thank you for your reply and for clarifying the questions.

    I can confirm that both these antibodies (ab693 and ab134093) are tested and covered by our 6 month guarantee for flow cytometry.

    However, I am sorry they have not been tried and tested for FACS cell sorting where live cells are required, so regrettably we would not be able to guarantee how well this will work. Both contain low concentration of preservative which may affect the cell viability.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

    Read More

    Question

    bonjour,
    non ce n'est pas du tout cette adresse; lubio est notre fournisseur, et nous a envoyé vos produits. l'adresse est:

    Read More
    Answer

    Merci pour ces précisions.

    J'aimerais vous informer que nous n'avons aucun accord commercial avec LuBioScience et que ce revendeur n'est pas en droit de distribuer nos produits. La garantie Abpromise d'Abcam ne s'applique que sur les produits commandés directement chez Abcam ou via un distributeur agréé (https://www.abcam.com/abpromise).

    Je m'engage cependant à faire exception pour le cas présent et accepte de vous envoyer gratuitement une unité de ab14879 directement à votre laboratoire comme convenu précédemment. Le numéro de la commande est ******.

    Pourriez-vous, s'il vous plait, placer vos futures commandes directement chez Abcam et, si possible, informer vos collègues que la société LuBioScience n'est pas autorisée à revendre les produits Abcam.

    N'hésitez pas à m'informer des nouveaux résultats obtenus avec l'anticorps ab134093 et le lysat ab14879.

    Read More

    Question

    Bonjour,
    comme convenu, voici le questionnaire rempli ainsi qu'une phooto de mon blot, le problème étant que les controle positifs OVCAR3 et CA125 recombinant protein ne sont pas reconnu par l'anticorps:
    WB Questionnaire
    1) Abcam product code ab134093
    2) Abcam order reference number or product batch number: je ne l'ai pas à disposition, mais au nom de l'institut de police scientifique à Lausanne.
    3) Description of the problem : non reconnaissance de l’anticorps pour le lysat cellualire OVCAR3 qui m’ a été vendu par Novus comme étant un contrôle positif, et pour la protéine recombinante CA125 Partial Recombinant Protein de novus.
    4) Sample preparation:
    Type of sample (whole cell lysates, fraction, recombinant protein…): lysat celleulaire OVCAR3 et protéine recombinante CA125
    Lysis buffer: aucun
    Protease inhibitors: aucun
    Phosphatase inhibitors: aucun
    Reducing agent: Laemmli+mercaptoethanol
    Boiling for ≥5 min? yes
    Protein loaded ug/lane or cells/lane: 10ug
    Positive control: il s’agissait du controle positif
    Negative control: oui
    5) Percentage of gel: SDS-PAGE 7.5% precast, Biorad
    Type of membrane: PVDF-FL, 0.45u
    Protein transfer verified
    Blocking agent and concentration: Blck de Millipore
    Blocking time: 1 heure
    Blocking temperature: ambiante
    6) Primary antibody (If more than one was used, describe in
    “additional notes”):
    Concentration or dilution: 1/10000
    Diluent buffer: Blck de Millipore
    Incubation time: 1 heure
    Incubation temperature: ambiante
    7) Secondary antibody: Alexa Fluor 532 Goat anti-rabbit IgG
    Species: goat
    Reacts against: rabbit
    Concentration or dilution: 1/1000
    Diluent buffer: Blck de Millipore
    Incubation time: 1 heure
    Incubation temperature: ambiante
    Fluorochrome or enzyme conjugate: Alexa fluor 532
    8) Washing after primary and secondary antibodies: solution de lavage du WesternBreeze de life technologies
    Buffer: PBS
    Number of washes: 3x5min avec solution de lavage et 2x5min
    avec PBS ou eau
    9) Detection method: Laser 532 nm
    10) How many times have you run this staining? 3 fois
    Do you obtain the same results every time? oui

    Read More
    Answer

    Merci pour le questionnaire.

    Nous n'avons pour l'instant en stock que le lot GR93682 pour l'anti-MUC16 ab134093.
    J'ai été informé par mes collègues du laboratoire que le peptide bloquant est disponible à la vente mais que la production de celui-ci prendrait quelques semaines.

    Afin de comprendre et déterminer l'origine du problème rencontré, je peux vous proposer de mettre en place l'envoi d'une unité de lysat nucléaire NIH OVCAR-3 référence ab14879 (https://www.abcam.com/ab14879). Ce lysat a été utilisé comme contrôle positif pour l'anti-MUC16 ab134093 dilué à 1/50,000 en conditions dénaturantes.
    Alternativement je peux vous envoyer une unité d'un anti-MUC16 monoclonal de souris tel que le ab1107 (https://www.abcam.com/ab1107) ou le ab10033 (https://www.abcam.com/ab10033).

    Merci de m'indiquer la résolution qui vous conviendrait le mieux.

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    Question

    lysat cellule OVECAR3
    protein recombinante ne fonctionne pas

    Read More
    Answer

    Merci pour votre appel. Je suis très désolée que nous n'avions pas répondu après votre premier appel - je ne sais pas ce qui c'est passé.

    Veuillez trouver ci-jointle questionnaire que nous avons discutéau téléphonequi nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps.
    Celui-ci nous permettra de vous fournir la meilleure assistance technique.

    Si nous concluons que notre produit est fautif, nous allons vous le remplacer.

    Comme discuté au téléphone, je vais aussi contacter le laboratoire pour l'information des conditions WB ainsi que pour le peptide.

    Je vous remercie par avance de nous envoyer votre protocole.

    Read More

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