Recombinant
RabMAb

Recombinant Anti-MUC16 antibody [EPSISR23] (PerCP) (ab221280)

Overview

  • Product name

    Anti-MUC16 antibody [EPSISR23] (PerCP)
    See all MUC16 primary antibodies
  • Description

    Rabbit monoclonal [EPSISR23] to MUC16 (PerCP)
  • Host species

    Rabbit
  • Conjugation

    PerCP. Ex: 482nm, Em: 675nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MUC16 aa 12150-12250. The exact sequence is proprietary.
    Database link: Q8WX17
    (Peptide available as ab139976)

  • Positive control

    • Flow Cyt: SK-OV-3 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • Tissue specificity

    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • Sequence similarities

    Contains 2 ANK repeats.
    Contains 56 SEA domains.
  • Domain

    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • Post-translational
    modifications

    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • Cellular localization

    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all

Images

  • Overlay histogram showing SK-OV-3 cells stained with ab221280 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221280, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20 mW blue solid-state laser (488nm) and >670 longpass filter.

References

ab221280 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221280.
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