• Product name
    Anti-MUC16 antibody [OC125]
    See all MUC16 primary antibodies
  • Description
    Mouse monoclonal [OC125] to MUC16
  • Host species
  • Specificity
    Studies have show that this antibody reacts with approximately 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell and undifferentiated types. No reactivity has been shown for mucinous ovarian tumors or in germ cell or hematopoietic tumors. It reacts with both normal tissues and neoplasms of fallopian tube, endometrium, endocervix and mesothelioma. It does not react with colon cancer. Normal tissues such as breast, liver, skin, kidney and spleen are negative.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Partially purified human mucin fraction from a pool of tissues from patients with epithelial ovarian cancer.

  • Epitope
    This antibody recognizes an epitope on a molecule called Cancer Antigen 125 (CA125).
  • Positive control
    • Flow cytometry: HeLa cells. IHC-P: Ovarian cancer tissue.



Our Abpromise guarantee covers the use of ab693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC Method.


  • Function
    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • Tissue specificity
    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • Sequence similarities
    Contains 2 ANK repeats.
    Contains 56 SEA domains.
  • Domain
    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • Post-translational
    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • Cellular localization
    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all


  • Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab693 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab693, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

  • Paraffin embedded human ovarian cancer tissue stained for MUC16 with ab693 (1/50 dilution) in immunohistochemical analysis.


This product has been referenced in:
  • Kline JB  et al. Tumor antigen CA125 suppresses antibody-dependent cellular cytotoxicity (ADCC) via direct antibody binding and suppressed Fc-? receptor engagement. Oncotarget 8:52045-52060 (2017). Flow Cyt ; Human . Read more (PubMed: 28881712) »
  • Kim BR  et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol 14:e1002581 (2016). ICC/IF ; Human . Read more (PubMed: 27880766) »
See all 11 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


Thank you for your interest in the MUC16 antibody, clone OC125. The papers from 1981 and 1983 that are listed in the references section of the ab693 datasheet describe production and characterization of the antibody. The antigen was not a peptide; it was a cell line, OVCA433, established from a patient with cystadenocarcinoma. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC370929/?page=2) The location of the epitope that the antibody recognizes has not, to our knowledge, been determined.

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The IgG concentration of the lot you have is 25.5 ug/mL. The antibody is not conjugated, so there is no F/P ratio.

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Thank you for your reply. The concentration for the current lot is 50.5 ug/mL. Please let me know if you have any further questions.

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Thank you for contacting us. The concentration is batch dependent. Have you already purchased the product? If so I can provide the concentration if you can send the lot number. Thank you!

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I am very pleased to hear you would like to accept our offer and test ab693 in IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Thank you for your reply and for clarifying the questions.

I can confirm that both these antibodies (ab693 and ab134093) are tested and covered by our 6 month guarantee for flow cytometry.

However, I am sorry they have not been tried and tested for FACS cell sorting where live cells are required, so regrettably we would not be able to guarantee how well this will work. Both contain low concentration of preservative which may affect the cell viability.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

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